The objective of the current study was to evaluate increasing levels of manganese hydroxychloride (MHC) in 45-wk-old white leghorn laying hens, using yolk and shell manganese (Mn) content as a potential marker for Mn concentration. A total of 80, 45-wk-old white leghorns were assigned to 6 dietary treatments, each consisting of 14 individually caged laying hens, with the exception of the reference diet containing 10 individually caged laying hens. The experiment consisted of a reference diet that contained 70 ppm of supplemental inorganic Mn in the form of Mn oxide and 5 experimental treatments each containing 0, 15, 30, 60, and 90 ppm supplemental MHC. Experimental birds were subjected to a 21 D depletion phase in which no supplemental Mn was included in the diet; however, during this time reference fed birds were fed the control diet (70 ppm Mn). After the 21 D depletion phase, the depleted birds were fed experimental diets for a 35 D evaluation period. Yolk and shell Mn content were analyzed at the end of the depletion phase and during the experimental phase on day 5, 10, 15, 25, and 35. During the experimental phase, Mn was replenished in the yolk and shell in all experimental treatments containing supplemental Mn; however, dose and time impacted the rate of replenishment. The yolk tended to be more sensitive to variations in Mn level as increases in Mn inclusion significantly (P < 0.05) increased concentration. These data demonstrate the ability to deplete and replenish Mn, and the use of egg yolk Mn concentration as measurement for determining changes in dietary Mn. At the conclusion of the experiment at 35 D, 60 ppm of Mn hydroxychloride seemed to be adequate in replenishing Mn to the level of the reference.

中文翻译：

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研究注：使用卵黄和壳中锰含量评估45周龄的白色来亨鸡层中的羟基氯化锰。

本研究的目的是使用卵黄和壳中锰（Mn）含量作为锰浓度的潜在标志物，评估45周龄的白色来格霍恩蛋鸡中羟基氯化锰（MHC）含量的增加。总共80头，45周龄的白色来亨鸡被分配给6种饮食处理方法，每种处理方法均由14只单独笼养的蛋鸡组成，但参考日粮中含有10只单独笼养的蛋鸡。该实验由参考饮食组成，该参考饮食包含70 ppm氧化锰形式的补充无机Mn和5种实验处理，每种处理均包含0、15、30、60和90 ppm的补充MHC。实验家禽处于21 D耗竭阶段，其中日粮中不包含补充Mn。但是，在这段时间内，参考饲喂的鸡只饲喂了对照饮食（70 ppm锰）。在21天的耗竭阶段之后，将耗竭的家禽喂食35天的评估期实验饲料。在耗竭阶段结束时和实验阶段的第5、10、15、25和35天分析蛋黄和蛋壳中的Mn含量。在实验阶段，在所有含有以下成分的实验处理中，蛋黄和蛋壳中的Mn被补充补充锰 但是，剂量和时间会影响补货速度。卵黄倾向于对锰水平的变化更敏感，因为锰含量的增加显着（P <0.05）增加了浓度。这些数据证明了补充和补充锰的能力，以及蛋黄中锰浓度作为确定膳食中锰含量变化的指标。在35 D实验结束时，