Heterogeneity of Streptococcus anginosus ß-hemolysis in relation to CRISPR/Cas.,Molecular Oral Microbiology

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Heterogeneity of Streptococcus anginosus ß-hemolysis in relation to CRISPR/Cas.
Molecular Oral Microbiology ( IF 2.8 ) Pub Date : 2020-02-06 , DOI: 10.1111/omi.12278
Richard Bauer ₁ , Nathalie Neffgen ₁ , Aline Grempels ₁ , Martina Furitsch ₁ , Stefanie Mauerer ₁ , Salome Barbaqadze ₂ , Gerhard Haase ₃ , Hans Kestler ₄ , Barbara Spellerberg ₁

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Streptococcus anginosus is a commensal of the oral mucosa that can cause severe invasive infections. A considerable proportion of Streptococcus anginosus strains are ß‐hemolytic due to the presence of an SLS‐like gene cluster. However, the majority of strains do not display ß‐hemolysis. To investigate ß‐hemolysin heterogeneity in S. anginosus, we determined the presence of sag genes and correlated it with the presence of CRISPR/Cas genes in a collection of ß‐hemolytic and non‐ß‐hemolytic strains. All of the ß‐hemolytic strains carried the sag gene cluster. In contrast to other streptococci, clinical S. anginosus strains that do not display ß‐hemolysis do not harbor sag genes. Phylogenetic analysis of the ß‐hemolytic strains revealed that they belong to two previously defined clusters within S. anginosus. Correlation with CRISPR/Cas genes showed a significant difference for the presence of CRISPR/Cas in ß‐hemolytic versus non‐ß‐hemolytic isolates. The presence of the CRISPR/Cas type IIA or type IIC locus is associated with the absence of sag genes; in 65% of the non‐ß‐hemolytic strains a CRISPR/Cas locus was found, while only 24% of ß‐hemolytic strains carry CRISPR/Cas genes. Further analysis of the spacer content of the CRISPR systems revealed the presence of multiple self‐targeting sequences directed against S. anginosus genes. These results support the hypothesis that horizontal gene transfer is involved in the acquisition of ß‐hemolysin genes and that CRISPR/Cas may limit DNA uptake in S. anginosus.

中文翻译：

链球菌链球菌β-溶血与CRISPR / Cas的异质性。

链球菌性心绞痛是口腔粘膜的征兆，可引起严重的侵袭性感染。由于存在类似SLS的基因簇，相当大比例的链球菌链球菌可被溶血。但是，大多数菌株不显示ß-溶血作用。为了研究链球菌中β-溶血素的异质性，我们确定了sag基因的存在并将其与CRISPR / Cas基因的存在相关联，该集合存在于β-溶血性和非β-溶血性菌株中。所有β-溶血菌株均携带sag基因簇。与其它链球菌，临床S.咽峡炎链球菌菌株与不显示β-内溶血不怀有下垂基因。对ß-溶血菌株的系统进化分析表明，它们属于链球菌中两个先前定义的簇。与CRISPR / Cas基因的相关性显示ß/溶血分离株与非ß-溶血分离株中CRISPR / Cas的存在显着差异。CRISPR / Cas IIA型或IIC型基因座的存在与sag基因的缺乏有关。在65％的非ß-溶血菌株中，发现了CRISPR / Cas基因座，而只有24％的ß-溶血菌株带有CRISPR / Cas基因。对CRISPR系统中间隔子含量的进一步分析表明存在多个针对心绞痛链球菌的自我靶向序列基因。这些结果支持以下假设：水平基因转移与β-溶血素基因的获取有关，而CRISPR / Cas可能会限制链球菌中DNA的摄取。

更新日期：2020-02-06

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