Pancreatic cancer, an extremely aggressive malignancy, is resistant to chemo- or radiotherapy. The rapid progression of pancreatic cancer without distinctive clinical sign makes early diagnosing and/or treating very difficult. BAF45D, a member of the d4 domain family, is involved in oncogenic processes. However, the role of BAF45D in pancreatic tumorigenesis is largely unclear. Our goal is to examine BAF45D protein expression after lentivirus-mediated Baf45d RNAi and explore the effects of BAF45D knockdown on cell proliferation, cell apoptosis, and cell cycle of human pancreatic cancer cells. Here our results showed that Baf45d RNAi downregulated BAF45D protein levels and decreased cell viability, increased cell apoptosis, and decreased colony formation in BxPC-3 cells. Moreover, BAF45D knockdown induced S-phase arrest in BxPC-3 cells. Our results here suggest that BAF45D may play a crucial role in tumorigenic properties of human pancreatic cancer cells.

中文翻译：

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BAF45D组合式降低人类胰腺癌细胞的细胞活力，抑制集落形成，诱导细胞凋亡和S期阻滞。

胰腺癌是一种极具侵略性的恶性肿瘤，对化学疗法或放射疗法有抵抗力。没有明显的临床体征的胰腺癌的快速发展使得早期诊断和/或治疗非常困难。BAF45D是d4结构域家族的成员，参与致癌过程。但是，BAF45D在胰腺肿瘤发生中的作用尚不清楚。我们的目标是检查慢病毒介导的Baf45d RNAi后BAF45D蛋白的表达，并探讨BAF45D敲低对人胰腺癌细胞的增殖，细胞凋亡和细胞周期的影响。在这里，我们的结果表明，Baf45d RNAi下调了BxPC-3细胞中BAF45D的蛋白水平，降低了细胞活力，增加了细胞凋亡，并减少了菌落形成。此外，BAF45D组合式诱导BxPC-3细胞中的S期停滞。