Nongonadal tissues express luteinizing hormone-chorionic gonadotropin receptors (LHCG-R) which are essential for their growth during fetal development. Adult mesenchymal stem/stromal cells (MSCs) have been shown to express functional LHCG-R outside pregnancy conditions, making them susceptible to hCG stimulation. In the present study we tested the effect of hCG treatment on bone marrow (BM) derived adherent stem cells in vitro, isolated from a parous women, mother of male sons, in order to evaluate its effect on maternal MSCs and in the same time on fetal microchimeric stem cells (FMSCs), to better understand the outcomes of this safe and affordable treatment on cell proliferation and expression of pluripotency genes. Our study highlights the beneficial effects of hCG exposure on gene regulation in bone marrow adherent stem cells through the upregulation of pluripotency genes and selection of more primitive mesenchymal stem cells with a better differentiation potential. Validation of these effects on MSCs and FMSCs long after parturition in vivo represents a close perspective as it could set the premises of a new mobilization strategy for the stem cell transplantation procedures in the clinical setting.

中文翻译：

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人绒毛膜促性腺激素可体外改善骨髓粘附干细胞的增殖和再生潜能以及胎儿微嵌合干细胞的免疫耐受性。

非性腺组织表达促黄体生成激素的绒毛膜促性腺激素受体（LHCG-R），这对它们在胎儿发育过程中的生长至关重要。成人间充质干/基质细胞（MSC）已显示在怀孕条件之外表达功能性LHCG-R，使其易于受到hCG刺激。在本研究中，我们测试了hCG治疗对体外源自骨髓（BM）的贴壁干细胞的作用，为了评估其对母体间充质干细胞以及同时对胎儿微嵌合干细胞（FMSC）的影响，从产妇中分离，以评估其对细胞增殖和表达的安全性和可承受性多能性基因。我们的研究强调了hCG暴露通过多能性基因的上调和选择具有更好分化潜能的更原始的间充质干细胞对骨髓粘附干细胞基因调控的有益影响。在体内分娩后很长时间验证这些对MSC和FMSC的作用代表了一个紧密的观点，因为它可以为临床环境中干细胞移植程序的新动员策略奠定前提。