We used a new multimodal imaging system that combines optical coherence microscopy and brightfield microscopy. Using this in vivo brain monitoring approach and cranial window implantation, we three-dimensionally visualized the vascular network during thrombosis, with high temporal (18 s) and spatial (axial, 2.5    μ m ; lateral, 2.2    μ m ) resolution. We used a modified mouse model of photochemical thromboembolic stroke in order to more accurately parallel human stroke. Specifically, we applied green laser illumination to focally occlude a branch of the middle cerebral artery. Despite the recanalization of the superficial arteries at 24 h after stroke, no blood flow was detected in the small vessels within deeper regions. Moreover, after 24 h of stroke progression, scattering signal enhancement was observed within the stroke region. We also evaluated the infarct extent and shape histologically. In summary, we present a novel approach for real-time mouse brain monitoring and ischemic variability analysis. This multimodal imaging method permits the analysis of thrombosis progression and reperfusion. Additionally and importantly, the system could be used to study the effect of poststroke drug treatments on blood flow in small arteries and capillaries of the brain.

中文翻译：

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在血栓栓塞性光化学中风的小鼠模型中使用多模式光学相干显微镜进行体内脑成像。

我们使用了一个新的多模态成像系统，该系统结合了光学相干显微镜和明场显微镜。使用这种体内脑监测方法和颅窗植入术，我们可以在血栓形成过程中以三维方式可视化血管网络，并具有较高的时间分辨率（18 s）和空间分辨率（轴向为2.5μm；横向为2.2μm）。为了更准确地平行人类中风，我们使用了改良的光化学血栓栓塞性中风小鼠模型。具体来说，我们应用了绿色激光照明来聚焦闭塞大脑中动脉的分支。尽管卒中后24 h浅表动脉再通，但在较深区域的小血管中未检测到血流。而且，在中风进行24小时后，在中风区域内观察到散射信号增强。我们还通过组织学评估了梗塞程度和形状。总而言之，我们提出了一种用于实时鼠标大脑监测和缺血性变异性分析的新颖方法。这种多峰成像方法可以分析血栓形成的进展和再灌注。此外，重要的是，该系统可用于研究中风后药物治疗对大脑小动脉和毛细血管中血流的影响。