BACKGROUND Pigmentation development, is a complex process regulated by many transcription factors during development. With the development of the RNA sequencing (RNA-seq), non-coding RNAs, such as miRNAs, lncRNAs, and circRNAs, are found to play an important role in the function detection of related regulation factors. In this study, we provided the expression profiles and development of ncRNAs related to melanocyte and skin development in mice with black coat color skin and mice with white coat color skin during embryonic day 15 (E15) and postnatal day 7 (P7). The expression profiles of different ncRNAs were detected via RNA-seq and also confirmed by the quantitative real-time PCR (qRT-PCR) method. GO and KEGG used to analyze the function the related target genes. RESULTS We identified an extensive catalogue of 206 and 183 differently expressed miRNAs, 600 and 800 differently expressed lncRNAs, and 50 and 54 differently expressed circRNAs, respectively. GO terms and pathway analysis showed the target genes of differentially expressed miRNA and lncRNA. The host genes of circRNA were mainly enriched in cellular process, single organism process. The target genes of miRNAs were mainly enriched in chromatin binding and calcium ion binding in the nucleus. The function of genes related to lncRNAs are post translation modification. The competing endogenous RNA (ceRNA) network of lncRNAs and circRNAs displays a complex interaction between ncRNA and mRNA related to skin development, such as Tcf4, Gnas, and Gpnms related to melanocyte development. CONCLUSIONS The ceRNA network of lncRNA and circRNA displays a complex interaction between ncRNA and mRNA related to skin development and melanocyte development. The embryonic and postnatal development of skin provide a reference for further studies on the development mechanisms of ncRNA during pigmentation.

中文翻译：

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全面检测miRNA、lncRNA、circRNA对小鼠黑素细胞和皮肤发育的调控作用。

背景技术色素沉着的形成是一个在发育过程中受多种转录因子调控的复杂过程。随着RNA测序（RNA-seq）的发展，人们发现非编码RNA，如miRNA、lncRNA和circRNA在相关调控因子的功能检测中发挥着重要作用。在本研究中，我们提供了黑毛色皮肤小鼠和白毛色皮肤小鼠在胚胎第 15 天 (E15) 和出生后第 7 天 (P7) 期间与黑素细胞和皮肤发育相关的 ncRNA 的表达谱和发育情况。通过RNA-seq检测不同ncRNA的表达谱，并通过定量实时PCR（qRT-PCR）方法确认。利用GO和KEGG分析相关靶基因的功能。结果我们分别确定了 206 和 183 种不同表达的 miRNA、600 种和 800 种不同表达的 lncRNA、以及 50 种和 54 种不同表达的 circRNA 的广泛目录。GO term和pathway分析显示差异表达的miRNA和lncRNA的靶基因。circRNA的宿主基因主要富集于细胞过程、单一生物体过程。miRNA的靶基因主要富集于核内染色质结合和钙离子结合。lncRNA相关基因的功能是翻译后修饰。lncRNA和circRNA的竞争性内源RNA（ceRNA）网络显示出ncRNA和与皮肤发育相关的mRNA之间复杂的相互作用，例如与黑素细胞发育相关的Tcf4、Gnas和Gpnms。结论 lncRNA 和 circRNA 的 ceRNA 网络显示出与皮肤发育和黑素细胞发育相关的 ncRNA 和 mRNA 之间复杂的相互作用。皮肤的胚胎和产后发育为进一步研究色素沉着过程中ncRNA的发育机制提供参考。