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Identification and Characterization of Fungi Contaminated in the Built-In Furniture of an Apartment Home.
Mycobiology ( IF 1.9 ) Pub Date : 2019-12-26 , DOI: 10.1080/12298093.2019.1703529
Min Ah Choi 1 , Geum Ran Ahn 1 , Seong Hwan Kim 1
Affiliation  

Fungal contamination of built-in furniture is a frequent problem in Korea when new apartment is built. However, domestic information on the contaminating fungi is very limited. This study was conducted to isolate, identify and characterize the fungi of the problem in one of the apartment houses where the fungi were claimed in the built-in furniture before the house owner moves in. Fungi present in the furniture installed in a main room, dress room, and kitchen side were visually and microscopically confirmed and purely isolated on PDA. The isolated fungi were identified by analyzing the morphological characteristics and nucleotide sequence of the ITS, calmodulin gene, and TEF-1α gene. Aspergillus creber, A. niger, A. pseudoglacus, A. ruber, Cladosporium perangustum and Penicillium commune were identified. Four out of the six fungal species were positive for at least one enzyme in six kinds of extracellular enzyme assays. When these four species (A. creber, A. niger, C. perangustum and P. commune) were inoculated onto four kinds of wood chips of furniture materials, they were able to colonize all of the wood chips. Their settlement was better at 95% humidity condition than at 30% humidity condition. Among the four species, C. perangustum caused the darkest discoloration and secreted the most number of extracellular enzymes. The four species were re-isolated from the colonized wood chips and confirmed as the problematic fungi in the built-in furniture.



中文翻译:

鉴定和表征公寓住宅的内置家具中污染的真菌。

在韩国建造新公寓时,内置家具的真菌污染是一个经常出现的问题。但是,有关污染真菌的国内信息非常有限。进行这项研究是为了隔离,识别和表征问题的真菌,其中一间公寓房在房主搬进来之前已在内置家具中对真菌进行了声明。该真菌存在于安装在主房间的家具中,更衣室和厨房侧均通过肉眼和显微镜确认,并在PDA上完全隔离。通过分析ITS,钙调蛋白基因和TEF-1α基因的形态特征和核苷酸序列来鉴定分离的真菌。黑克里伯黑曲霉A. pseudoglacusA.曲霉,鉴定了桔梗青霉公社。在六种细胞外酶测定中,六种真菌中的四种对至少一种酶呈阳性。当将这四个物种(A. creber,A。niger,C。perangustumP. commune)接种到四种家具材料的木片上时,它们便能够在所有木片上定殖。在湿度为95%时,其沉降要比湿度为30%时好。在四个物种中,C。perangustum引起最深的变色,并分泌最多的细胞外酶。从定殖的木片中重新分离出这四种,并确认它们是内置家具中有问题的真菌。

更新日期:2019-12-26
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