The hospital environment requires special attention to air quality, since it needs to be healthy for the protection of patients and health professionals in order to prevent them against hospital infections. The objective of this study was to isolate, identify and evaluate the susceptibility profile of isolated fungi from two hospitals. For air sampling the impaction (Spin Air, IUL^®) and passive sedimentation methods were used. For the isolation of fungi from surfaces, contact plates (RODAC^®) were used. The identification of the fungi was performed by observing the macroscopic and microscopic aspects of the colonies, whereas for better visualization of fruiting structures, the microculture technique was performed on slides. To evaluate the susceptibility profile, the broth microdilution test recommended by CLSI was performed. Thirty-five isolates were identified: Aspergillus flavus (12), Aspergillus fumigatus (11), Aspergillus niger (1), Aspergillus terreus (2), Penicillium spp. (7), and Fusarium spp. (2) in the hospitals evaluated. All isolates had a minimum inhibitory concentration (MIC) more than 128 μg/ml for fluconazole; 0.5 to 4.0 μg/ml for amphotericin B (hospital 1), and all isolates from haospital 2 had MIC ≥2.0 μg/ml. In hospital 1, MIC for posaconazole ranged from 0.25 μg/ml to ≥32 μg/ml, and hospital 2 ranged from 0.5 to 1.0 μg/ml. The monitoring and evaluation of air quality and surfaces are essential measures for prevention and control of hospital infections, as these microorganisms are becoming increasingly resistant to antimicrobial agents, thus making treatment difficult, especially in immunocompromised individuals.

中文翻译：

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从医院的手术中心，重症监护室和材料灭菌中心分离出的致病真菌相关的风险。从关键医院区域隔离的致病真菌的风险。

医院环境需要特别注意空气质量，因为它需要健康以保护患者和卫生专业人员，以防止他们受到医院感染。这项研究的目的是分离，鉴定和评估两家医院分离出的真菌的药敏性。用于空气采样嵌塞（自旋空气，IUL ^®）和无源沉降方法被使用。用于从表面，接触板真菌的隔离（RODAC ^®）。真菌的鉴定是通过观察菌落的宏观和微观方面进行的，而为了更好地可视化结实的结构，对载玻片进行了微培养技术。为了评估药敏曲线，进行了CLSI推荐的肉汤微稀释试验。鉴定出三十五种分离物：黄曲霉（12），烟曲霉（11），黑曲霉（1），土曲霉（2），青霉菌。（7）和镰刀菌spp。（2）在医院进行评估。所有分离物对氟康唑的最低抑菌浓度（MIC）均大于128μg/ ml；两性霉素B（医院1）的浓度为0.5至4.0μg/ ml，而来自医院2的所有分离物的MIC≥2.0μg/ ml。在医院1中，泊沙康唑的MIC范围为0.25μg/ ml至≥32μg/ ml，医院2的MIC范围为0.5至1.0μg/ ml。空气质量和表面的监测和评估是预防和控制医院感染的重要措施，因为这些微生物对抗菌剂的抵抗力越来越强，从而使治疗变得困难，尤其是对免疫功能低下的人。