Abstract

Capillary electrochromatography (CEC) is a powerful hybrid separation technique that combines capillary electrophoresis and capillary chromatography, capable to address the analytical challenges of proteomics and glycomics. The focus of this paper is to review the recent developments in capillary electrochromatography of proteins and carbohydrates. The different column types applied in capillary electrochromatography such as packed bed, open tubular and monoliths are conferred in detail with respective separation examples. A comprehensive comparison is also given listing the mostly utilized coating methods, stationary phase materials and column preparation methods. The choice of porogenic solvent combinations for monolithic column fabrication is thoroughly discussed, paying close attention to the fine tuning options for the separation driving electroosmotic flow. Application examples of CEC in process analytical technology for the biopharmaceutical and biomarker discovery in the biomedical fields are also given.

摘要

毛细管电色谱 (CEC) 是一种强大的混合分离技术，它结合了毛细管电泳和毛细管色谱，能够解决蛋白质组学和糖组学的分析挑战。本文的重点是回顾蛋白质和碳水化合物毛细管电色谱的最新发展。毛细管电色谱中应用的不同色谱柱类型，如填充床、开管和整体柱，在各自的分离实例中进行了详细介绍。还给出了综合比较，列出了最常用的涂层方法、固定相材料和柱制备方法。彻底讨论了用于整体柱制造的致孔溶剂组合的选择，密切关注分离驱动电渗流的微调选项。还给出了 CEC 在过程分析技术中的应用实例，用于生物医学领域的生物制药和生物标志物发现。