This study aims to evaluate the CD146+ stem cells obtained from the human umbilical cord and their extracellular matrix proteins on in vitro Pseudomonas aeruginosa and Staphylococcus aureus biofilms to understand their possible antimicrobial activity. CD146+ stem cells were determined according to cell surface markers and differentiation capacity. Characterization of the decellularized matrix was done with DAPI, Masson’s Trichrome staining and proteome analysis. Cell viability/proliferation of cells in co-cultures was evaluated by WST-1 and crystal-violet staining. The effects of cells and decellularized matrix proteins on biofilms were investigated on a drip flow biofilm reactor and their effects on gene expression were determined by RT-qPCR. We observed that CD146/105+ stem cells could differentiate adipogenically and decellularized matrix showed negative DAPI and positive collagen staining with Masson’ s Trichrome. Proteome analysis of the decellularized matrix revealed some matrix components and growth factors. Although the decellularized matrix significantly reduced the cell counts of P. aeruginosa, no significant difference was observed for S. aureus cells in both groups. Supporting data was obtained from the gene expression results of P. aeruginosa with the significant down-regulation of rhlR and lasR. For S. aureus, icaADBC genes were significantly up-regulated when grown on the decellularized matrix.

中文翻译：

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人脐带 CD146+ 干细胞和基于干细胞的脱细胞基质上金黄色葡萄球菌和铜绿假单胞菌生物膜形成的评估。

本研究旨在评估从人脐带获得的 CD146+ 干细胞及其细胞外基质蛋白对体外铜绿假单胞菌和金黄色葡萄球菌生物膜的作用，以了解它们可能的抗菌活性。根据细胞表面标志物和分化能力来确定CD146+干细胞。通过 DAPI、Masson 三色染色和蛋白质组分析对脱细胞基质进行表征。通过 WST-1 和结晶紫染色评估共培养物中细胞的活力/增殖。在滴流生物膜反应器上研究了细胞和脱细胞基质蛋白对生物膜的影响，并通过 RT-qPCR 测定了它们对基因表达的影响。我们观察到CD146/105+干细胞可以分化为脂肪，脱细胞基质显示DAPI阴性和Masson's Trichrome胶原染色阳性。脱细胞基质的蛋白质组分析揭示了一些基质成分和生长因子。尽管脱细胞基质显着减少了铜绿假单胞菌的细胞计数，但两组中金黄色葡萄球菌细胞没有观察到显着差异。从铜绿假单胞菌基因表达结果中获得了支持数据，其中rhlR和lasR显着下调。对于金黄色葡萄球菌，icaADBC基因在脱细胞基质上生长时显着上调。