INTRODUCTION Frequently observed multiple sensitizations to several animals highlights the importance of a molecular diagnosis, distinguishing between sensitizations specific to single species and sensitizations due to cross-reactivity. OBJECTIVE The aim of our study was to assess the usefulness of a molecular diagnosis in the description of sensitization profiles in allergy patients living in Central Europe, with a particular focus on animal-derived molecules. METHODS The molecular diagnosis was performed using the ImmunoCAP ISAC microarray. Results of 1,255 allergy patients were subjected to statistical analysis. RESULTS The highest sensitization rates were observed for uteroglobin Fel d 1 (31.8%) and kallikrein Can f 5 (16.4%), followed by animal lipocalins Can f 1 (13.9%), Equ c 1 (6.2%), Fel d 4 (5.3%), Can f 2 (4.2%), and Mus m 1 (4.1%). Sensitization rates to serum albumins Fel d 2, Can f 3, Equ c 3, and Bos d 6 were very low, with the highest being 3.2% to Fel d 2. Detailed subanalysis confirmed the dominant role of Fel d 1 or Can f 5 and/or Can f 1 in cat- or dog-sensitized patients, respectively. Further analysis focused on lipocalins and albumins confirmed a high rate of cosensitizations within both groups. CONCLUSION The sensitization to animal allergen molecules is very frequent in Central Europe. The most common is sensitization to species-specific cat uteroglobin Fel d 1 and dog kallikrein Can f 5, followed by sensitizations to animal lipocalins. Our data suggest that commonly observed multiple sensitizations detected by extract approach can be explained not only by true cosensitization, but also by cross-reactivity, mainly in the frame of lipocalins. Cross-reactive serum albumins are minor sensitizers and are probably not important from this point of view.

中文翻译：

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中欧过敏症患者的致敏特性分析主要针对动物过敏原分子。

引言经常观察到的对几种动物的多重敏化突出了分子诊断的重要性，区分了对单个物种特有的敏化和由于交叉反应而引起的敏化。目的我们研究的目的是评估分子诊断在描述中欧过敏性患者致敏性档案中的有用性，尤其侧重于动物衍生的分子。方法使用ImmunoCAP ISAC芯片进行分子诊断。对1,255名过敏患者的结果进行了统计分析。结果对子宫珠蛋白Fel d 1（31.8％）和激肽释放酶Can f 5（16.4％）的增敏率最高，其次是动物脂钙素Can f 1（13.9％），Equ c 1（6.2％），Fel d 4（ 5.3％），Can f 2（4.2％）和Mus m 1（4.1％）。对血清白蛋白Fel d 2，Can f 3，Equ c 3和Bos d 6的敏感率非常低，最高为Fel d 2的3.2％。详细的亚分析证实了Fel d 1或Can f 5的主导作用。和/或Can f 1分别对猫或狗敏感的患者。聚焦于脂蛋白和白蛋白的进一步分析证实了两组中的高致敏率。结论在中欧对动物过敏原分子的致敏非常频繁。最常见的是对物种特异性猫子宫珠蛋白Fel d 1和犬激肽释放酶Can f 5的致敏作用，然后是对动物脂蛋白的致敏作用。我们的数据表明，提取物方法检测到的普遍观察到的多种敏化现象，不仅可以通过真正的共敏化得到解释，而且还可以通过交叉反应来解释，主要是在脂蛋白的框架中。