Salicylic acid (SA) and nitric oxide (NO) are considered as putative plant growth regulators that are involved in the regulation of an array of plant's growth and developmental functions under environmental fluctuations when applied at lower concentrations. The possible involvement of NO in SA induced attenuation of high temperature (HT) induced oxidative stress in plants is however, still vague and need to be explored. Therefore, the present study aimed to investigates the biochemical and physiological changes induced by foliar spray of SA and NO combinations to ameliorate HT induced oxidative stress in Lablab purpureus L. Foliar application of combined SA and NO significantly improved relative water content (27.8 %), photosynthetic pigment content (67.2 %), membrane stability (45 %), proline content (1.0 %), expression of enzymatic antioxidants (7.1-18 %) along with pod yield (1.0 %). Heat Shock Factors (HSFs) play crucial roles in plants abiotic stress tolerance, however there structural and functional classifications in L. purpureus L. is still unknown. So, In-silico approach was also used for functional characterization and homology modelling of HSFs in L. purpureus. The experimental findings depicted that combine effect of SA and NO enhances tolerance in HT stressed L. purpureus L. plants by regulating physiological functions, antioxidants, expression and regulation of stress-responsive genes via transcriptional regulation of heat shock factor.

中文翻译：

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水杨酸和一氧化氮在转录重编程中对紫癜的高温耐受性的相互作用：利用计算方法对结构和功能的见解。

水杨酸（SA）和一氧化氮（NO）被认为是推定的植物生长调节剂，当以较低浓度施用时，它们在环境波动下参与一系列植物的生长和发育功能的调节。但是，NO可能参与SA诱导的植物高温（HT）诱导的氧化应激衰减的抑制作用仍很模糊，需要加以研究。因此，本研究旨在研究叶面喷洒SA和NO组合物以减轻Lablab purpureus L中HT诱导的氧化应激所引起的生化和生理变化。叶面喷施SA和NO组合物可显着提高相对含水量（27.8％），光合色素含量（67.2％），膜稳定性（45％），脯氨酸含量（1.0％），酶抗氧化剂的表达（7.1-18％）以及豆荚产量（1.0％）。热休克因子（HSF）在植物非生物胁迫耐受性中起着关键作用，但是紫杉L.的结构和功能分类仍然未知。因此，In-silico方法也被用于紫癜L. HSFs的功能表征和同源性建模。实验结果表明，SA和NO的结合作用通过热休克因子的转录调控来调节生理功能，抗氧化剂，应激反应基因的表达和调控，从而增强了HT胁迫的紫苏植物的耐受性。仍然未知。因此，In-silico方法也被用于紫癜L. HSFs的功能表征和同源性建模。实验结果表明，SA和NO的结合作用通过热休克因子的转录调控来调节生理功能，抗氧化剂，应激反应基因的表达和调控，从而增强了HT胁迫的紫苏植物的耐受性。仍然未知。因此，In-silico方法也被用于紫癜L. HSFs的功能表征和同源性建模。实验结果表明，SA和NO的结合作用通过热休克因子的转录调控来调节生理功能，抗氧化剂，应激反应基因的表达和调控，从而增强了HT胁迫的紫苏植物的耐受性。