S-adenosyl-l-methionine (SAM) has been attracting increasing attention because of its significance in the pharmaceutical industry; however, the high cost of this compound limits its application. Tofu yellow serofluid exhibits high nutritional value and is not costly; therefore, it can be utilized as a substrate in the fermentation industry. In the current study, Saccharomyces cerevisiae was cultured in the tofu yellow serofluid fermentation medium for the SAM biosynthesis. The optimum tofu yellow serofluid fermentation medium contained 70 g/L of glucose, 30 % of yellow serofluid, 20 g/L of l-methionine, and 2.5 g/L of ammonium citrate. Under these conditions, the optimum feeding strategy was established. The results revealed that the dry cell weight (DCW) reached 123.1 g/L, the maximum production of SAM was 16.14 g/L, the highest SAM productivity reached 1.048 g/L/h, and SAM content was determined at 131.1 mg/g DCW. Furthermore, addition of tofu yellow serofluid reduced the average cost of SAM by 31.9 % to compare with the culture process without addition of tofu yellow serofluid. Thus, the tofu yellow serofluid fermentation medium improved the production of SAM and significantly reduced the production costs.

中文翻译：

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使用豆腐黄色浆液改善酿酒酵母中S-腺苷-1-蛋氨酸的生产。

S-腺苷-1-蛋氨酸（SAM）由于其在制药工业中的重要性而受到越来越多的关注。但是，这种化合物的高成本限制了其应用。豆腐黄色浆液具有很高的营养价值，而且价格不贵；因此，它可用作发酵工业的底物。在当前的研究中，酿酒酵母在豆腐黄浆液发酵培养基中培养，用于SAM的生物合成。最佳的豆腐黄色浆液发酵培养基包含70 g / L的葡萄糖，30％的黄色浆液，20 g / L的L-蛋氨酸和2.5 g / L的柠檬酸铵。在这些条件下，确定了最佳喂养策略。结果表明，干细胞重量（DCW）达到123.1 g / L，SAM的最大产量为16.14 g / L，SAM的最高生产率达到1.048 g / L / h，SAM含量确定为131.1 mg / g DCW。此外，与不添加豆腐黄色浆液的培养过程相比，添加豆腐黄色浆液可将SAM的平均成本降低31.9％。因此，豆腐黄色浆液发酵培养基改善了SAM的生产并显着降低了生产成本。