Respiration and Heat Shock Protein After Short-Term Heating/Stretch-Fixing on Smooth Muscle Cells.,Cardiovascular Engineering and Technology

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Respiration and Heat Shock Protein After Short-Term Heating/Stretch-Fixing on Smooth Muscle Cells.
Cardiovascular Engineering and Technology ( IF 1.6 ) Pub Date : 2020-02-03 , DOI: 10.1007/s13239-020-00458-y
Nao Kaminota ₁ , Emiyu Ogawa ₂ , Hiroshi Kumagai ₂ , Kosuke Tsukada _{1,

3} , Tsunenori Arai ₃

Affiliation

Purpose

A treatment device without a stent is needed for peripheral stenotic artery treatment. We have proposed short-term heating balloon angioplasty, photo-thermo dynamic balloon angioplasty (PTDBA). Though smooth muscle cells (SMCs) after PTDBA are fixed in a stretched formation in a porcine model, influences of this stimulus on SMCs have not been investigated. SMC migration after vascular dilatation would be related to chronic restenosis. The aim of this study was to examine respiratory activity and recovery ability of SMCs after short-term heating/stretch-fixing in vitro for chronic phase treatment effect discussion.

Methods

SMCs on a stretch chamber were heated for 15 s with stretching and fixed in a stretched formation. SMC migration is correlated with the cell respiratory activity. The amount of ATP production was measured using a WST-8 assay for respiratory activity evaluation. The intracellular expression of heat shock protein 70 was measured by an ELISA for recovery ability evaluation.

Results

In the case of 60 °C heating, SMC respiratory activity after short-term heating/stretch-fixing decreased drastically in all stretching rates. In the case of 50 °C heating, SMC respiratory activity decreased and then increased. Alternatively, the recovery ability at 60 °C was greater than that at 50 °C.

Conclusions

SMCs heated at 60 °C with stretching would have high recovery ability and low respiratory activity related to SMC migration. These results may be important evidence in determining the treatment condition in PTDBA.

中文翻译：

短期加热/拉伸固定在平滑肌细胞上后的呼吸和热休克蛋白。

目的

外周狭窄动脉的治疗需要没有支架的治疗装置。我们提出了短期加热球囊血管成形术，光热动力球囊血管成形术（PTDBA）。尽管在猪模型中PTDBA之后的平滑肌细胞（SMC）固定为伸展形式，但尚未研究这种刺激对SMC的影响。血管扩张后SMC迁移可能与慢性再狭窄有关。这项研究的目的是在体外进行短期加热/固定固定后检查SMC的呼吸活动和恢复能力，以讨论慢性期治疗效果。