In this study, the effects of Aspergillus niger in coculture with the basidiomycetes, Trametes versicolor, T. maxima, and Ganoderma spp., were studied to assess H₂O₂ production and laccase (Lac), Lignin Peroxidase (LiP), and manganese peroxidase (MnP) activities. The results indicated that maximum discoloration was of 97%, in the T. maxima and A. niger coculture, where the concentration of H₂O₂ was 5 mg/L and 6.3 mg/L in cultures without and with dye, respectively. These concentrations of H₂O₂ were 1.6- and 1.8-fold higher than monocultures of T. maxima (3.37 mg/L) and A. niger (3.87 mg/L), respectively. In the same coculture, the LiP and MnP enzyme activities also increased 12-fold, (from 0.08 U/mg to 0.99 U/mg), and 67-fold, (from 0.11 U/mg to 7.4 U/mg), respectively. The Lac activity increased 1.7-fold (from 13.46 U/mg to 24 U/mg). Further, a Box–Behnken experimental design indicated a 1.8-fold increase of MnP activity (from 7.4 U/mg to 13.3 U/mg). In addition, dye discoloration regression model obtained from the Box–Behnken experimental design showed a positively correlation with H₂O₂, (R² = 0.58) and a negatively correlation with Lac activity (R² = –0.7).

在这项研究中，研究了黑曲霉与担子菌，Trametes versicolor，T。maxim a和Ganoderma spp。共培养的效果，以评估H ₂ O _2的产生以及漆酶（Lac），木质素过氧化物酶（LiP）和锰过氧化物酶（MnP）活性。结果表明，最大的变色是97％，在T.最大值和黑曲霉共培养，其中H的浓度₂ ö ₂为5mg / L和不具有和具有染料，分别为6.3毫克/升培养物。这些H ₂ O ₂浓度分别比最大T.（3.37 mg / L）和A. niger（3.87 mg / L）的单培养高1.6倍和1.8倍。在相同的共培养物中，LiP和MnP酶活性也分别增加了12倍（从0.08 U / mg到0.99 U / mg）和67倍（从0.11 U / mg到7.4 U / mg）。Lac活性增加了1.7倍（从13.46 U / mg增至24 U / mg）。此外，Box-Behnken实验设计表明MnP活性增加了1.8倍（从7.4 U / mg增至13.3 U / mg）。另外，从Box–Behnken实验设计获得的染料褪色回归模型显示与H ₂ O ₂正相关（R ²  = 0.58），与Lac活性负相关（R ² = –0.7）。