Dietary polyunsaturated fatty acids affect volume and metabolism of Drosophila melanogaster sperm,Journal of Evolutionary Biology

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Dietary polyunsaturated fatty acids affect volume and metabolism of Drosophila melanogaster sperm
Journal of Evolutionary Biology ( IF 2.1 ) Pub Date : 2020-02-03 , DOI: 10.1111/jeb.13591
Ruijian Guo ₁ , Klaus Reinhardt ₁

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Dietary fatty acids can accumulate in sperm and affect their function in vertebrates. As Drosophila melanogaster shares several pathways of lipid metabolism and shows similar lipid‐dependent phenotypes but lacks some hormones that in vertebrates regulate lipid metabolism, there is currently no clear prediction as to how dietary fatty acids affect the sperm of D. melanogaster. We manipulated the amount and identity of dietary polyunsaturated fatty acids (PUFA) in the food of D. melanogaster males (a treatment known to affect membrane fluidity) and measured changes in sperm parameters. We found that (a) males reared on food containing PUFA‐rich, plant‐derived lipids showed a slower increase in sperm volume over male age compared to males reared on yeast‐derived lipid food which is richer in saturated fatty acids. (b) The resistance of sperm membrane integrity to osmotic stress was not altered by dietary lipid treatment, but (c) food containing yeast‐derived lipids induced a 46% higher in situ rate of production of reactive oxygen species in sperm cells. These findings show that dietary lipids have similar effects on sperm parameters in Drosophila as in vertebrates, affect some, but not all, sperm parameters and modulate male reproductive ageing. In concert with recent findings of sex‐specific seasonal variation of diet choice in the wild, our results suggest a substantial dietary impact on the dynamics of male reproduction in the wild.

中文翻译：

膳食多不饱和脂肪酸影响黑腹果蝇精子的体积和代谢

膳食脂肪酸可以在精子中积累并影响它们在脊椎动物中的功能。由于 Drosophila melanogaster 共享几种脂质代谢途径并显示出相似的脂质依赖性表型，但缺乏脊椎动物中调节脂质代谢的一些激素，因此目前尚无明确预测膳食脂肪酸如何影响 D. melanogaster 的精子。我们操纵了黑腹果蝇雄性食物中膳食多不饱和脂肪酸 (PUFA) 的数量和特性（一种已知会影响膜流动性的处理方法），并测量了精子参数的变化。我们发现 (a) 与使用富含饱和脂肪酸的酵母源性脂质食物饲养的雄性相比，以富含 PUFA 的植物性脂质食物饲养的雄性在超过雄性年龄时表现出精子体积的缓慢增加。(b) 精子膜完整性对渗透胁迫的抵抗力未因饮食脂质处理而改变，但 (c) 含有酵母衍生脂质的食物使精子细胞中活性氧的原位产生率提高了 46%。这些发现表明，膳食脂质对果蝇精子参数的影响与脊椎动物相似，会影响部分（但不是全部）精子参数并调节雄性生殖衰老。与最近关于野外饮食选择的性别特定季节性变化的发现一致，我们的结果表明饮食对野外雄性繁殖的动态有重大影响。这些发现表明，膳食脂质对果蝇精子参数的影响与脊椎动物相似，会影响部分（但不是全部）精子参数并调节雄性生殖衰老。与最近关于野外饮食选择的性别特定季节性变化的发现一致，我们的结果表明饮食对野外雄性繁殖的动态有重大影响。这些发现表明，膳食脂质对果蝇精子参数的影响与脊椎动物相似，会影响部分（但不是全部）精子参数并调节雄性生殖衰老。与最近关于野外饮食选择的性别特定季节性变化的发现一致，我们的结果表明饮食对野外雄性繁殖的动态有重大影响。

更新日期：2020-02-03

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