This paper described how a high-yield, monodisperse Au nanobipyramids (Au NBs) sol was prepared by a seed-mediated method, and gold nanoparticles were assembled on the surface of a silicon wafer by self-assembly technology to obtain a solid SERS substrate. Scanning electron microscopy (SEM) showed that the average length of Au NBs was 34.31 nm, and the analysis enhancement factor (AEF) was approximately 7.3 × 10⁵ with rhodamine 6G (R6G) used as a probe. SERS detection of chlortetracycline hydrochloride (CCH) in milk was performed utilizing the prepared Au NBs substrate, and the limit of detection was 0.01 mg/mL. In the range of 0.01 – 1 mg/mL, the mass concentration of CCH and the SERS signal intensity satisfied the linear relationship y = 258.467x + 150.501; the value of the correlation coefficient was 0.9785. In addition, the recovery of spiked samples fluctuated between 96.80 to 111.38%. These results proved that the method is simple and fast, and it is promising to be applied to the field detection of antibiotics in milk.

本文介绍了如何通过种子介导的方法制备高产率、单分散的金纳米双锥体（Au NBs）溶胶，并通过自组装技术将金纳米粒子组装在硅片表面以获得固体SERS基底。扫描电子显微镜（SEM）显示，以罗丹明6G（R6G）为探针，Au NBs的平均长度为34.31 nm，分析增强因子（AEF）约为7.3×10 ^{5 。}利用制备的Au NBs底物对牛奶中的盐酸金霉素(CCH)进行SERS检测，检测限为0.01 mg/mL。在0.01~1mg/mL范围内，CCH质量浓度与SERS信号强度满足线性关系y =258.467x + 150.501；相关系数值为0.9785。此外，加标样品的回收率在96.80%至111.38%之间波动。结果表明，该方法简单、快速，有望应用于牛奶中抗生素的现场检测。