We previously demonstrated that a simple modification in the preparation of agar media, i.e., autoclaving phosphate and agar separately (termed the “PS protocol”), improved the culturability of aerobic microorganisms by reducing the generation of reactive oxygen species. We herein investigated the effects of the PS protocol on the cultivation of anaerobic microorganisms using sludge from a wastewater treatment system as a microbial source. The application of the PS protocol increased colony numbers and the frequency of phylogenetically novel isolates under aerobic, nitrate reduction, and fermentation conditions. The PS protocol is useful for isolating both aerobic and anaerobic microorganisms.

中文翻译：

---

使用琼脂和磷酸盐单独灭菌制备的培养基改进未培养厌氧细菌的分离

我们之前已经证明，在制备琼脂培养基时进行简单的修改，即分别对磷酸盐和琼脂进行高压灭菌（称为“PS 方案”），通过减少活性氧的产生来提高需氧微生物的可培养性。我们在此研究了 PS 协议对使用来自废水处理系统的污泥作为微生物源培养厌氧微生物的影响。PS 协议的应用在有氧、硝酸盐还原和发酵条件下增加了菌落数量和系统发育新分离物的频率。PS 协议可用于分离需氧和厌氧微生物。