Various Long non-coding RNAs (lncRNAs) and MicroRNAs (miRNAs) have been demonstrated to be involved in colorectal cancer stem cells (CSCs). WBSCR22 is a key gene we previously found that functions in colorectal cancer (CRC). This paper aims to investigate the effects of WBSCR22 and its corresponding miRNA and lncRNA in CRC. The expression of WBSCR22 was detected by Western blot and qRT-PCR analysis. Tumor sphere formation assays, CCK-8 analysis, and Transwell assays were applied to examine the colorectal cancer stem cell properties. Luciferase assay and biotin RNA pulldown assay were used to detect the interaction between miR-509-5p and WBSCR22/Linc00346. WBSCR22 is highly expressed in CRC and correlates with poor prognosis. Knockdown of WBSCR22 decreased the sphere-forming capacities, cell proliferation, and invasion abilities. WBSCR22 was negatively regulated by miR-509-5p. Linc00346 promoted the expression of WBSCR22 by adsorbing miR-509-5p. The Linc00346/miR-509-5p/WBSCR22 signal axis regulated the characteristics of colon cancer stem cells. Linc00346 regulated the expression of WBSCR22 by binding to miR-509-5p, thereby monitoring the characteristics of colorectal cancer stem cells. Thus, WBSCR22, Linc00346, and miR-509-5p might be utilized as potential targets for clinical diagnosis and treatment of CRC.

中文翻译：

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WBSCR22与长的非编码RNA Linc00346竞争miR-509-5p结合位点，以调节结直肠癌的癌症干细胞表型。

已证明各种长非编码RNA（lncRNA）和MicroRNA（miRNA）参与结直肠癌干细胞（CSC）。WBSCR22是我们先前发现在结肠直肠癌（CRC）中起作用的关键基因。本文旨在研究WBSCR22及其相应的miRNA和lncRNA在CRC中的作用。通过蛋白质印迹和qRT-PCR分析检测WBSCR22的表达。肿瘤球形成测定，CCK-8分析和Transwell测定用于检查结直肠癌干细胞特性。使用萤光素酶测定法和生物素RNA下拉测定法检测miR-509-5p与WBSCR22 / Linc00346之间的相互作用。WBSCR22在CRC中高表达，并且与不良预后相关。击倒WBSCR22降低了球形成能力，细胞增殖和侵袭能力。WBSCR22受miR-509-5p负调控。Linc00346通过吸附miR-509-5p促进WBSCR22的表达。Linc00346 / miR-509-5p / WBSCR22信号轴调节结肠癌干细胞的特性。Linc00346通过与miR-509-5p结合来调节WBSCR22的表达，从而监测结直肠癌干细胞的特性。因此，WBSCR22，Linc00346和miR-509-5p可作为CRC临床诊断和治疗的潜在靶标。