当前位置: X-MOL 学术Struct. Dyn. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Evolving data standards for cryo-EM structures.
Structural Dynamics ( IF 2.3 ) Pub Date : 2020-01-24 , DOI: 10.1063/1.5138589
Catherine L Lawson 1 , Helen M Berman , Wah Chiu
Affiliation  

Electron cryo-microscopy (cryo-EM) is increasingly being used to determine 3D structures of a broad spectrum of biological specimens from molecules to cells. Anticipating this progress in the early 2000s, an international collaboration of scientists with expertise in both cryo-EM and structure data archiving was established (EMDataResource, previously known as EMDataBank). The major goals of the collaboration have been twofold: to develop the necessary infrastructure for archiving cryo-EM-derived density maps and models, and to promote development of cryo-EM structure validation standards. We describe how cryo-EM data archiving and validation have been developed and jointly coordinated for the Electron Microscopy Data Bank and Protein Data Bank archives over the past two decades, as well as the impact of evolving technology on data standards. Just as for X-ray crystallography and nuclear magnetic resonance, engaging the scientific community via workshops and challenging activities has played a central role in developing recommendations and requirements for the cryo-EM structure data archives.

中文翻译:

不断发展的冷冻电镜结构数据标准。

冷冻电子显微镜 (cryo-EM) 越来越多地用于确定从分子到细胞的广泛生物样本的 3D 结构。预计 2000 年代初将取得这一进展,在冷冻电镜和结构数据归档方面拥有专业知识的科学家组成的国际合作组织成立了(EMDataResource,以前称为 EMDataBank)。此次合作的主要目标有两个:开发必要的基础设施来归档冷冻电镜衍生的密度图和模型,并促进冷冻电镜结构验证标准的开发。我们描述了过去二十年来如何开发和联合协调电子显微镜数据库和蛋白质数据库档案的冷冻电镜数据归档和验证,以及不断发展的技术对数据标准的影响。正如 X 射线晶体学和核磁共振一样,通过研讨会和具有挑战性的活动吸引科学界参与,在制定冷冻电镜结构数据档案的建议和要求方面发挥了核心作用。
更新日期:2020-01-24
down
wechat
bug