The quality of bone marrow preparation and sections of teeth depends on the preparation method. We investigated the posterior mandibles of male rats that were processed using a modified Bouin’s solution (MBS) and stained with Harris’ hematoxylin and eosin, and the results were compared to a routine decalcification process using 5% formal nitric acid. We found that MBS was applicable to both soft and hard components in bone tissues and sections of teeth. MBS provided good decalcification, which facilitated sectioning. The bone marrow specimens treated with MBS exhibited clearly distinguishable hematopoietic cells, clear tissue integrity and good cell preservation. Treatment with 5% formal nitric acid for 5 days caused degeneration, poor differentiation and poorer staining of hematopoietic components, and deteriorated soft and solid tissues in bone and sections of teeth compared to MBS treatment. MBS preserved cell and tissue integrity and good staining quality. MBS can be used for rapid preparation and diagnosis for pathology and toxicity studies.

骨髓制备和牙齿切片的质量取决于制备方法。我们调查了使用改良的布因溶液（MBS）处理并用Harris's苏木精和曙红染色的雄性大鼠的后下颌，并将其与使用5％甲醛的常规脱钙过程进行了比较。我们发现MBS适用于骨骼组织和牙齿部分的软硬成分。MBS提供了良好的脱钙效果，有助于切片。用MBS处理的骨髓标本显示出明显可区分的造血细胞，清晰的组织完整性和良好的细胞保存性。用5％甲醛溶液处理5天会导致变性，分化不良和造血成分染色变差，与MBS治疗相比，骨骼和牙齿的软硬组织变质。MBS保留了细胞和组织的完整性以及良好的染色质量。MBS可用于病理学和毒性研究的快速制备和诊断。