Here we evaluate the potential of anterograde gaseous oxygen persufflation for graft reconditioning after extended storage times. Pig livers were retrieved and cold-stored in HTK solution for 16 h. Some grafts were subsequently subjected to anterograde gaseous oxygen persufflation via the portal vein for 2 h. Oxygen concentrations for persufflation were either 100% or 40%. The gas was insufflated at a pressure adjusted to 18 mmHg, a pressure required to see gas bubbles leaving at the hepatic vein. Gas flow required for adequate maintenance of persufflation pressure amounted to approx. 300 ml/min in both groups. Only the use of 100% oxygen resulted in a significant increase of end-ischemic tissue ATP and improved bile flow upon reperfusion. Brief anterograde oxygen persufflation can improve energetic status of ischemic livers prior to transplantation, but the use of pure oxygen and adequate gas flow seems necessary to improve ulterior graft function.

中文翻译：

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顺行肝灌注氧浓度对缺血保存后高能磷酸盐及移植物功能的影响

在这里，我们评估了在延长储存时间后顺行气态氧渗透用于移植物修复的潜力。取回猪肝并在 HTK 溶液中冷藏 16 小时。一些移植物随后通过门静脉进行顺行气态氧灌注 2 小时。用于灌注的氧气浓度为 100% 或 40%。在调节至 18 mmHg 的压力下吹入气体，这是看到气泡离开肝静脉所需的压力。充分维持灌注压力所需的气体流量约为 两组均为 300 毫升/分钟。仅使用 100% 氧气会导致缺血末期组织 ATP 显着增加并改善再灌注时的胆汁流量。