Chemosensory avoidance behaviors of aquatic invertebrates provide a functional link between early responses to pollutants at the infraorganismal and ecologically relevant supraorganismal levels. Despite significant importance, there is, however, a notable lack of user-friendly laboratory techniques. Here, we demonstrate a scalable millifluidic platform for higher throughput quantitative chemobehavioral studies. With a proof-of-concept application of this technology, we discovered that native Australian marine amphipods Allorchestes compressa exhibit rapid avoidance behaviors against a panel of environmental stressors. This work provides a novel avenue for the development of quantitative neurobehavioral systems applicable in diverse environmental risk assessment studies.

中文翻译：

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使用两流体灌流技术揭示了海洋两栖动物的变种Allorchestes compressa的化学感觉回避行为。

水生无脊椎动物的化学感应回避行为提供了在无机生物和生态相关的超有机生物水平上对污染物的早期反应之间的功能联系。尽管非常重要，但是仍然明显缺乏用户友好的实验室技术。在这里，我们展示了可扩展的微流体平台，用于更高通量的定量化学行为研究。通过这项技术的概念验证应用，我们发现澳大利亚本土的海洋两栖类双足小扁豆对一组环境压力因子表现出快速回避行为。这项工作为可应用于各种环境风险评估研究的定量神经行为系统的开发提供了一条新途径。