ABSTRACT

In this study, we aimed to study the effect of fluid shear stress on fibroblasts and BMSCs on plane and groove topographies. The results showed that 0.6-Hz stress had the greatest influence on the alignment, polarity, migration and adhesion of fibroblasts on plane by increasing the expression of reoriented actin and vinculin; whereas 1.0-Hz stress promoted differentiation of fibroblasts into myofibroblasts by increasing Col-I and α-SMA expression. Interestingly, under the given frequency stress, the groove structure strengthened the above characteristics of fibroblasts beyond adhesion, and promoted differentiation of BMSCs into myofibroblasts. The above results indicate that 0.6 Hz may improve the implant-tissue sealing, while 1.0-Hz stress probably causes the disordered fiber deposition around implants.

摘要

在这项研究中，我们旨在研究在平面和凹槽形貌上流体剪切应力对成纤维细胞和BMSCs的影响。结果表明，0.6 Hz应力通过增加重新定向的肌动蛋白和纽蛋白的表达，对成纤维细胞在平面上的排列，极性，迁移和粘附具有最大的影响。而1.0 Hz压力通过增加Col-I和α-SMA表达促进成纤维细胞分化为成肌纤维细胞。有趣的是，在给定的频率应力下，凹槽结构增强了成纤维细胞的上述特性，使其不粘附，并促进了BMSCs向成肌纤维细胞的分化。以上结果表明，0.6 Hz可能会改善植入物与组织之间的密封，而1.0 Hz的应力可能会导致植入物周围的纤维沉积紊乱。