A serological survey of 2,430 archived serum samples collected between 1997 and 2012 was conducted to retrospectively determine the prevalence of Marburg virus in five African countries. Serum samples were screened for neutralizing antibodies in a pseudotype micro-neutralization assay and confirmed by enzyme-linked immunosorbent assay (ELISA). Surprisingly, a seroprevalence for Marburg virus of 7.5 and 6.3% was found in Cameroon and Ghana, respectively, suggesting the circulation of filoviruses or related viruses outside of known endemic areas that remain undetected by current surveillance efforts. However, due to the lack of validated assays and appropriate positive controls, these results must be considered preliminary.

中文翻译：

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在西非和中非针对马尔堡或相关丝状病毒的血清反应。

对1997年至2012年收集的2,430份存档血清样本进行了血清学调查，以追溯确定马尔堡病毒在五个非洲国家的流行情况。在假型微中和测定中筛选血清样品中和抗体，并通过酶联免疫吸附测定（ELISA）进行确认。令人惊讶的是，在喀麦隆和加纳发现的马尔堡病毒血清阳性率分别为7.5％和6.3％，这表明目前已知的监测工作仍未发现已知流行地区以外的丝状病毒或相关病毒的传播。但是，由于缺乏经过验证的检测方法和适当的阳性对照，因此这些结果必须视为初步的。