Rapid urbanization, industrial activity, and runoff have all played a role in transforming the Anacostia River from a biologically rich ecosystem to an ecologically threatened environment facing extensive pollution. In recent decades, numerous groups have worked to document and begin to address pollution in the waterway, but few have examined the biological impact of these contaminants. To assess water quality, the current study examined the effects of Anacostia water on early fish development and behavior using zebrafish (Danio rerio). Zebrafish embryos and larvae were reared in water samples collected from the Washington Navy Yard from 0-30dpf (days post fertilization). At 7, 15, 20, and 30dpf, larvae were subsampled for morphological (length, girth, eye diameter, inter-eye distance) and behavioral (angular velocity, total distance traveled, swimming velocity, total activity duration, time immobile, frequency and duration of burst swimming, time at the edge of the dish) assessment. Water samples were processed using gas chromatography-mass spectroscopy (GC-MS) to identify major organic contaminants. Results indicated the presence of 13 bioactive organic contaminants, including siloxane species and hormone derivatives, and accelerated growth and altered swim behaviors in Anacostia-exposed fish after 30 days of exposure. These findings emphasize sublethal but significant impacts of exposure to organic contaminants experienced by fish residing in urban waterways.

中文翻译：

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Anacostia河水样品中饲养的幼体斑马鱼（Danio rerio）的解剖和行为评估。

快速的城市化，工业活动和径流都在将Anacostia河从生物丰富的生态系统转变为面临广泛污染的生态威胁环境中发挥了作用。在最近的几十年中，许多小组致力于记录并开始解决水道中的污染问题，但是很少有人研究这些污染物的生物学影响。为了评估水质，当前的研究使用斑马鱼（Danio rerio）检查了Anacostia水对鱼类早期发育和行为的影响。将斑马鱼的胚胎和幼虫放在0-30dpf（受精后的几天）从华盛顿海军院收集的水样中饲养。在7、15、20和30dpf时，分别对幼虫进行形态学（长度，周长，眼睛直径，眼间距离）和行为（角速度，总移动距离，游泳速度，总活动时间，不动时间，爆发游泳的频率和持续时间，菜边缘的时间）评估。使用气相色谱-质谱法（GC-MS）处理水样品以鉴定主要的有机污染物。结果表明，暴露30天后，暴露于Anacostia的鱼中存在13种生物活性有机污染物，包括硅氧烷种类和激素衍生物，并加速了生长并改变了游泳行为。这些发现强调了居住在城市水道中的鱼类接触到有机污染物所致的致命性，但影响显着。使用气相色谱-质谱法（GC-MS）处理水样品以鉴定主要的有机污染物。结果表明，暴露30天后，暴露于Anacostia的鱼中存在13种生物活性有机污染物，包括硅氧烷种类和激素衍生物，并且加速了生长并改变了游泳行为。这些发现强调了居住在城市水道中的鱼类接触到有机污染物所致的致命性，但影响显着。使用气相色谱-质谱法（GC-MS）处理水样品以鉴定主要的有机污染物。结果表明，暴露30天后，暴露于Anacostia的鱼中存在13种生物活性有机污染物，包括硅氧烷种类和激素衍生物，并且加速了生长并改变了游泳行为。这些发现强调了居住在城市水道中的鱼类接触到有机污染物所致的致命性，但影响显着。