Background: Under certain circumstances, the path for protein folding deviates and attains an alternative path forming misfolded states, which are the key precursors for protein aggregation. Protein aggregation is associated with variety of diseases and leads to the cytotoxicity. These protein aggregate related diseases have been untreated so far. However, extensive attempts have been applied to develop anti-aggregating agents as possible approaches to overcome protein aggregation. Different types of substances have been reported to halt or decrease the formation of ordered protein aggregates both in vitro and in vivo, such as polyphenols and metal ions.

Objective: In the present study the in vitro aggregation of human serum albumin (HSA) by using a reactive dicarbonyl glyoxal has been investigated, simultaneously an attempt has been done to inhibit the glyoxal (GO) induced aggregation of (HSA) by caffeic acid (CA).

Methods: Different methods have been employed to investigate the process, fluorescence spectroscopy, circular dichroism, cango red binding assay, thioflavin T dye binding, turbidimetric analysis, docking study and transmission electron microscopy.

Results: Results have shown that elevated concentration of GO forms aggregates of HSA, and the activity of CA suggested the possibility of inhibiting the HSA aggregation at higher concentrations, and this compound was found to have an anti-aggregation property.

Conclusion: The present study explained that micro molar concentrations of CA inhibits the aggregation of HSA and showed pronounced anti-aggregation effect at increasing concentrations in the presence of GO which is elevated in diabetic and hyperglycaemia conditions.

背景：在某些情况下，蛋白质折叠的路径会偏离并获得形成错误折叠状态的替代路径，这是蛋白质聚集的关键前体。蛋白质聚集与多种疾病有关，并导致细胞毒性。这些蛋白质聚集体相关疾病迄今尚未得到治疗。然而，已经进行了广泛的尝试来开发抗聚集剂，作为克服蛋白质聚集的可能方法。据报道，在体外和体内，不同类型的物质可阻止或减少有序蛋白质聚集体的形成，例如多酚和金属离子。

目的：在本研究中，研究了使用反应性二羰基乙二醛体外聚集人血清白蛋白（HSA）的方法，同时尝试抑制乙二醛（GO）诱导的咖啡酸（HSA）聚集（ CA）。

方法：已采用不同方法研究了该过程，荧光光谱，圆二色性，cango红结合测定，硫黄素T染料结合，比浊分析，对接研究和透射电子显微镜。

结果：结果表明，高浓度的GO会形成HSA的聚集体，而CA的活性暗示了在较高浓度下抑制HSA聚集的可能性，并且发现该化合物具有抗聚集特性。

结论：本研究解释说，微摩尔浓度的CA可抑制HSA的聚集，并在糖尿病和高血糖情况下GO升高时，浓度升高时显示出明显的抗聚集作用。