Brucellosis is a significant bacterial zoonotic disease with a high seroprevalence in low-to-middle-income countries where there is a significant contact of humans with animals. This prospective study was conducted to observe the seroprevalence of brucellosis in 75 symptomatic patients with pyrexia of unknown origin (PUO) and 75 high-risk individuals (10 veterinarians, 15 milkmen and 50 healthy contacts of symptomatic patients) with possible exposure to brucellosis. Serum samples collected from these patients and individuals were subjected to rose Bengal test (RBT) and ELISA for the detection of IgM and IgG antibodies. RBT was positive in 50 samples (40 PUO and 10 high risk), of which 25 (33.3%) PUO cases had detectable IgM antibodies, whereas IgG antibodies were detected in 20 patients. None of samples from high-risk individuals was reactive for IgM/IgG antibodies by ELISA. The overall seroprevalence in this study was 16.7% (33.3% in PUO patients). Being a predominantly livestock rearing area, brucellosis is a significant health-care problem in this part of India with this entity being linked to 33% of PUO cases.

中文翻译：

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拉贾斯坦邦西部布鲁氏菌病的血清阳性率：来自三级护理中心的一项研究。


布鲁氏菌病是一种重要的细菌性人畜共患疾病，在人与动物有大量接触的中低收入国家具有很高的血清流行率。这项前瞻性研究的目的是观察 75 名有症状且不明原因发热 (PUO) 的患者和 75 名可能接触布氏菌病的高危个体（10 名兽医、15 名送奶工和 50 名有症状患者的健康接触者）中布氏菌病的血清阳性率。从这些患者和个人收集的血清样本进行了玫瑰孟加拉试验（RBT）和 ELISA，以检测 IgM 和 IgG 抗体。 50 例（40 例 PUO 和 10 例高危）RBT 呈阳性，其中 25 例（33.3%）PUO 病例可检测到 IgM 抗体，而 20 例患者中检测到 IgG 抗体。通过 ELISA 检测，来自高危个体的样本均未对 IgM/IgG 抗体产生反应。本研究中的总体血清阳性率为 16.7%（PUO 患者为 33.3%）。作为一个以牲畜饲养为主的地区，布鲁氏菌病是印度这一地区的一个重大卫生保健问题，33% 的 PUO 病例与该疾病有关。