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Diagnostic performance of mini parasep® solvent-free foecal parasite concentrator for the diagnosis of intestinal parasitic infections.
Indian Journal of Medical Microbiology ( IF 1.4 ) Pub Date : 2019-01-01 , DOI: 10.4103/ijmm.ijmm_19_44
Abhishek Mewara 1 , Sumeeta Khurana 1 , Swati Gupta 2 , Vimal S Munda 2 , Shreya Singh 2 , Rakesh Sehgal 1
Affiliation  

Introduction Various stool concentration techniques have been used to increase the microscopic detection of parasites. We assessed the enclosed, single-vial, Mini Parasep® technique in comparison to the currently used coprodiagnosis procedures. Materials and Methods A total of 150 stool samples were collected. Samples concentrated by formol-ether method (FEM) and Mini Parasep® SF foecal concentrator and unconcentrated samples were subjected to wet mount, iodine mount microscopy and smear examination by modified acid-fast staining. Results Direct wet mount detected 72 positive samples (48.6%), whereas 77 (51.3%) and 80 (53.3%) samples were detected by FEM and Mini Parasep® SF methods, respectively. The sensitivity of detection of parasites was 98.7%, 95% and 90.1% with Mini Parasep®, FEM and direct microscopy, respectively. A clearer background with less foecal debris and a better yield of Hymenolepis nana, Trichuris trichiura, Entamoeba coli and Giardia lamblia were noted with Mini Parasep®. Conclusion Mini Parasep® SF technique is simple, rapid and less cumbersome than conventional diagnostics, making it suitable for routine use. In addition, it offers higher sensitivity and better background clearance than both direct stool examination and FEM.

中文翻译:


迷你 parasep® 无溶剂粪便寄生虫浓缩器用于诊断肠道寄生虫感染的诊断性能。



引言 各种粪便浓缩技术已被用来提高寄生虫的显微镜检出率。我们评估了封闭式单瓶 Mini Parasep® 技术与目前使用的粪便诊断程序的比较。材料和方法 总共收集了 150 份粪便样本。通过甲醛-乙醚法 (FEM) 和 Mini Parasep® SF 焦点浓缩器浓缩的样品和未浓缩的样品进行湿涂片、碘片显微镜检查并通过改良的抗酸染色进行涂片检查。结果 直接湿涂片检测到 72 个阳性样品 (48.6%),而 FEM 和 Mini Parasep® SF 方法分别检测到 77 个 (51.3%) 和 80 个 (53.3%) 样品。 Mini Parasep®、FEM 和直接显微镜检测寄生虫的灵敏度分别为 98.7%、95% 和 90.1%。 Mini Parasep® 具有更清晰的背景、更少的粪便碎片以及更高的膜壳虫、Trichuris trichiura、内阿米巴和贾第鞭毛虫产量。结论 Mini Parasep® SF 技术比传统诊断简单、快速且简便,适合常规使用。此外,与直接粪便检查和 FEM 相比,它具有更高的灵敏度和更好的背景清除率。
更新日期:2020-02-04
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