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Influence of selenium yeast on the growth, selenium uptake and mineral composition of Coriolus versicolor mushroom
Journal of Basic Microbiology ( IF 3.5 ) Pub Date : 2020-01-31 , DOI: 10.1002/jobm.201900520
Dunja Miletić 1 , Milena Pantić 1 , Aleksandra Sknepnek 1 , Ivana Vasiljević 2 , Milana Lazović 2 , Miomir Nikšić 1
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The ability of Coriolus versicolor medicinal mushroom to grow and accumulate selenium during submerged cultivation in a selenium‐fortified medium is examined in this paper. For selenium supplementation, commercial selenium yeast was used. Control, nonenriched sample and reference cultures cultivated in the medium enriched with commercial yeast Saccharomyces cerevisiae were also prepared. The mushroom demonstrated a high ability to accumulate selenium from the added source (around 970 and 1,300 µg/g of dry mycelium weight for samples enriched with selenium in a concentration of 10 and 20 mg Se/L, respectively). The addition of selenium significantly (p ≤ .05) increased the biomass yield, whereas the addition of nonenriched yeast had no significant (p ≤ .05) impact. Furthermore, regression analysis showed statistically significant (p ≤ .05) and positive correlations between the content of Se and Fe (r = .92), Se and Cu (r = .92), Se and Mn (r = .98), and Se and Sr (r = .96), suggesting that selenium incorporation was followed by incorporation of these elements, and led to mineral enrichment of the obtained mycelium. Methanol extracts prepared from mycelium biomass demonstrated a better inhibitory effect on Gram‐positive bacterial strains with minimal inhibitory concentrations between <0.3125 and 40 mg/ml. The obtained results showed that selenium yeast could be used for obtaining a potential novel food supplement: mushroom biomass with high selenium content and enhanced mineral composition.

中文翻译:

硒酵母对云芝生长、硒吸收及矿物质组成的影响

本文研究了云芝药用蘑菇在硒强化培养基中沉水培养过程中生长和积累硒的能力。对于硒补充,使用商业硒酵母。还制备了在富含商业酵母酿酒酵母的培养基中培养的对照、非浓缩样品和参考培养物。蘑菇表现出从添加的来源中积累硒的高能力(对于富含硒的样品,硒浓度分别为 10 和 20 毫克/升,干菌丝体重量分别约为 970 和 1,300 微克/克)。添加硒显着 (p ≤ .05) 增加了生物量产量,而添加非浓缩酵母没有显着 (p ≤ .05) 影响。此外,回归分析显示具有统计学意义(p ≤ . 05) 以及 Se 和 Fe (r = .92)、Se 和 Cu (r = .92)、Se 和 Mn (r = .98) 以及 Se 和 Sr (r = .96) 之间的正相关关系,表明硒掺入之后是这些元素的掺入,并导致获得的菌丝体的矿物质富集。从菌丝体生物质制备的甲醇提取物对革兰氏阳性细菌菌株具有更好的抑制作用,最小抑制浓度在 <0.3125 和 40 mg/ml 之间。获得的结果表明,硒酵母可用于获得潜在的新型食品补充剂:具有高硒含量和增强矿物质成分的蘑菇生物质。表明硒掺入之后是这些元素的掺入,并导致获得的菌丝体的矿物质富集。从菌丝体生物质制备的甲醇提取物对革兰氏阳性细菌菌株具有更好的抑制作用,最小抑制浓度在 <0.3125 和 40 mg/ml 之间。获得的结果表明,硒酵母可用于获得潜在的新型食品补充剂:具有高硒含量和增强矿物质成分的蘑菇生物质。表明硒掺入之后是这些元素的掺入,并导致获得的菌丝体的矿物质富集。从菌丝体生物质制备的甲醇提取物对革兰氏阳性细菌菌株具有更好的抑制作用,最小抑制浓度在 <0.3125 和 40 mg/ml 之间。获得的结果表明,硒酵母可用于获得潜在的新型食品补充剂:具有高硒含量和增强矿物质成分的蘑菇生物质。
更新日期:2020-01-31
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