Introduction

During recent decades, the emergence of chemoresistance among synthetic anthelmintic drugs has increased the interest in screening novel natural anthelmintic compounds derived from plants. The current study is aimed to determine the chemical profile, anthelmintic and antioxidant properties of Mentha pulegium hydro-ethanolic extract.

Materials and Methods

Two tests were used to assess the in vitro anthelmintic activity of the hydro-ethanolic extract of M. pulegium against Haemonchus contortus; egg hatch assay (EHA) and adult worm motility (AWM) assay. M. pulegium extracts at the doses of 500, 1000, 2000 and 4000 mg/kg were evaluated in vivo in mice infected with Heligmosomoides polygyrus. The anthelmintic efficacy was monitored using faecal egg count reduction (FECR) and total worm count reduction (TWCR). The antioxidant activity of M. pulegium extract was evaluated by testing the total antioxidant capacity and the DPPH free radical-scavenging ability.

Results

Chromatographic characterization of M. pulegium composition using RP-HPLC revealed the presence of phenolic acids such as syringic acid, ferulic acid and the presence of flavonoid compounds, such as isorhamnetin-3-O-glucoside and kaempferol-3-O-rutinoside. We observed 91.58% inhibition in the EHA at 8 mg/mL after 48 h of incubation (IC50=1.82 mg/mL). In the AWM assay, M. pulegium extract achieved 65.2% inhibition at 8 mg/mL after 8 h. The highest dose (4000 mg/kg) showed a significant nematicidal effect 7 days post-treatment by inducing 60.39% FECR and 71.6% TWCR. We also report strong in vivo antioxidant capacity of the extract, as revealed by a significant increase of the enzymatic activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes in mice infected with H. polygyrus.

Conclusion

Together, the results in this paper suggest that M. pulegium possesses anthelmintic properties and could be a potential source of novel compounds for the control of helminth parasites as well as its associated oxidative damage.

中文翻译：

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薄荷脑的水-乙醇提取物在体外和体内表现出驱虫和抗氧化特性。

介绍

在最近的几十年中，合成驱虫药中化学抗性的出现增加了人们对筛选源自植物的新型天然驱虫药的兴趣。当前的研究旨在确定薄荷薄荷水乙醇提取物的化学特征，驱虫和抗氧化特性。

材料和方法

两个测试被用来评估的水-乙醇提取物的体外驱虫活性M. pulegium对捻转血矛线虫; 卵孵化分析（EHA）和成虫蠕动分析（AWM）分析。在感染了多聚螺旋体的小鼠体内评估了浓度分别为500、1000、2000和4000 mg / kg的鼠疫支原体提取物。使用减少粪便卵数（FECR）和减少蠕虫总数（TWCR）来监测驱虫效果。通过测试总抗氧化能力和清除DPPH自由基的能力，评估了葛根提取物的抗氧化活性。

结果

使用RP-HPLC色谱法对鼠疫支原体组合物进行色谱表征，结果表明存在酚酸（如丁香酸，阿魏酸）和类黄酮化合物（如异鼠李素-3-O-葡萄糖苷和山奈酚-3- O-芸香苷）的存在。孵育48小时后，我们观察到在8 mg / mL的EHA中抑制了91.58％（IC50 = 1.82 mg / mL）。在AWM分析中，P。pulegium8小时后，提取物在8 mg / mL时达到65.2％的抑制率。最高剂量（4000 mg / kg）在治疗后7天表现出明显的杀线虫效果，诱导了60.39％的FECR和71.6％的TWCR。我们还报告了该提取物的强大的体内抗氧化能力，如在感染了H.polygyrus的小鼠中过氧化氢酶（CAT），超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）酶的酶活性显着增加所揭示的那样。

结论

在一起，本文的结果表明，鼠疫分枝杆菌具有驱虫特性，并且可能是控制蠕虫寄生虫及其相关氧化损伤的新型化合物的潜在来源。