Every year tens of millions reconstruction surgeries were performed to repair tissue defects caused by trauma or malignant tumor. As the composite tissues are very difficult to be preserved for a long time in vitro, "organ bank" was proposed. In addition to immune rejection, tissue cryopreservation is the key step of the "Organ Bank". In this study, two severed fingers were cryopreserved because the patients' conditions were not allowed to undergo the replantation immediately. The fingers were perfused with the cryopreservation solution through the digital artery at a speed of 1 ml/min, they were frozen following a control rate freezing procedure and stored in the liquid nitrogen. One finger was cryopreserved for 10 days and the other for 30 days. Both fingers were successfully replanted when the patient get better. After 1 year follow-up, the replanted fingers achieved satisfactory appearance and function. Our result demonstrate small composite tissues such as severed fingers can be cryopreserved and replanted successfully, which will help the patients who cannot receive the replantation immediately. This method will have important application value in cryobiology and clinical medicine.

中文翻译：

---

断指的冷冻保存与移植

每年进行数千万次重建手术，以修复因外伤或恶性肿瘤引起的组织缺损。由于复合组织在体外很难长期保存，因此提出了“器官库”。除了免疫排斥，组织冷冻保存是“器官库”的关键步骤。在这项研究中，由于患者的病情不允许立即进行再植，因此冷冻保存了两个断指。手指以1ml/min的速度通过手指动脉用冷冻保存液灌注，按照控制速率冷冻程序将它们冷冻并储存在液氮中。一根手指冷冻保存 10 天，另一根手指冷冻保存 30 天。当病人好转时，两个手指都成功地再植。1年后随访，再植手指获得满意的外观和功能。我们的结果表明，断指等小的复合组织可以成功冷冻保存和再植，这将有助于不能立即接受再植的患者。该方法将在低温生物学和临床医学中具有重要的应用价值。