Irradiation is a physical, environmentally friendly treatment which does not leave any residues in the product. It is increasingly used as an alternative to methyl bromide and other chemical fumigants for disinfestation of insect pest in stored grains. In this research, we try to evaluate the effect of low gamma irradiation doses in the range of 50–1200 Gy on the different stages of Callosobruchus maculatus. It was found that no adults emerged after irradiation of eggs at 450 Gy. No emerging adults (F1 generation) have been shown when larvae or pupae were exposed to 650 Gy. Also, no emerging adult has been shown from the parental C. maculatus adults irradiated with 650 Gy. When suppression of F1 generation was used for the measuring effective irradiation dose and phytosanitary efficacy, 650 Gy was required for disinfestation of cowpea seed weevils. The irradiation dose level of 650 Gy was used for the large-scale confirmatory tests applied to 27,754 adults of C. maculatus in cowpea seeds resulting in non F1 adults' production with a confidence level of 93.77%. All the physical and chemical characteristics of cowpea seeds were non-significantly (P ≤ 0.05) affected by the irradiation dose of 650 Gy. Meanwhile, this irradiation dose very slightly reduced the microbial load of cowpea seeds. We recommend the generic dose 650 Gy as the phytosanitary irradiation dose for the cowpea seed weevil.

中文翻译：

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评估辐照对黄豆豇豆不同发育阶段和豇豆种子化学、物理和微生物质量的影响

辐照是一种物理的、环保的处理方法，不会在产品中留下任何残留物。它越来越多地用作甲基溴和其他化学熏蒸剂的替代品，用于杀灭储存谷物中的害虫。在这项研究中，我们试图评估 50-1200 Gy 范围内的低伽马辐射剂量对不同阶段的影响。斑蝾螈. 发现在 450 Gy 照射鸡蛋后没有成虫出现。当幼虫或蛹暴露于 650 Gy 时，未显示出成虫（F1 代）。此外，没有从父母那里看到新生的成年人C. maculatus成年人接受 650 Gy 的辐照。当使用抑制 F1 代来测量有效辐照剂量和植物检疫效力时，豇豆种子象鼻虫的除害需要 650 Gy。650 Gy 的辐照剂量水平用于对 27,754 名成年人进行的大规模验证性试验。C. maculatus在豇豆种子中导致非 F1 成虫产量的置信水平为 93.77%。豇豆种子各项理化特性均不显着（磷≤ 0.05) 受 650 Gy 辐照剂量的影响。同时，这种辐照剂量非常轻微地降低了豇豆种子的微生物负荷。我们推荐通用剂量 650 Gy 作为豇豆种子象鼻虫的植物检疫辐照剂量。