Objective: Amylases enzymes hydrolyze starch molecules to produce diverse products including dextrins, and progressively smaller polymers. These include glucose units linked through α-1- 1, α-1-4, α-1-6, glycosidic bonds.

Methods: This enzyme carrying an (α /β) 8 or TIM barrel structure is also produced containing the catalytic site residues. These groups of enzymes possess four conserved regions in their primary sequence. In the Carbohydrate-Degrading Enzyme (CAZy) database, α-amylases are classified into different Glycoside Hydrolase Families (GHF) based on their amino acid sequence. The present objective was to study one such enzyme based on its molecular characterization after purification in our laboratory. Its main property of solid-natural starch degradation was extensively investigated for its pharmaceutical/ industrial applications.

Results: Amylase producing bacteria Bacillus cereus sm-sr14 (Accession no. KM251578.1) was purified to homogeneity on a Seralose 6B-150 gel-matrix and gave a single peak during HPLC. MALDITOF mass-spectrometry with bioinformatics studies revealed its significant similarity to α/β hydrolase family. The enzyme showed an efficient application; favourable Km, Vmax and Kcat during the catalysis of different natural solid starch materials. Analysis for hydrolytic product showed that this enzyme can be classified as the exo-amylase asit produced a significant amount of glucose.

Conclusion: Besides the purified enzyme, the present organism Bacillus cereus sm-sr14 could degrade natural solid starch materials like potato and rice up to the application level in the pharmaceutical/ industrial field for alcohol production.

目的：淀粉酶可水解淀粉分子以产生多种产品，包括糊精和逐渐变小的聚合物。这些包括通过α-1-1，α-1-4，α-1-6，糖苷键连接的葡萄糖单元。

方法：还生产出带有（α/β）8或TIM桶状结构的酶，其中含有催化位点残基。这些酶组在其一级序列中具有四个保守区。在碳水化合物降解酶（CAZy）数据库中，α-淀粉酶根据其氨基酸序列分为不同的糖苷水解酶家族（GHF）。本发明的目的是基于在我们实验室中纯化后基于其分子特征研究一种这样的酶。对其固体-天然淀粉降解的主要特性进行了广泛的研究，以用于其制药/工业应用。

结果：将产生淀粉酶的蜡样芽胞杆菌sm-sr14（登录号KM251578.1）在Seralose 6B-150凝胶基质上纯化至均一，并在HPLC中给出一个单峰。具有生物信息学研究的MALDITOF质谱分析表明，其与α/β水解酶家族具有显着相似性。该酶显示出有效的应用。在不同的天然固体淀粉材料的催化过程中，有利的Km，Vmax和Kcat。对水解产物的分析表明，由于产生大量葡萄糖，该酶可以归类为外淀粉酶。

结论：除了纯化的酶外，目前的蜡样芽孢杆菌sm-sr14还可以降解马铃薯和大米等天然固体淀粉原料，在制药/工业生产酒精中的应用水平最高。