Introduction: Glycoproteomics is an important subdiscipline of proteomics, focusing on the role of protein glycosylation in various biological processes. Protein glycosylation is the enzymatic addition of sugars or oligosaccharides to proteins. Altered glycosylation often occurs in the early stages of disease development, for example, certain tumor-associated glycans have been shown to be expressed in precursor lesions of different types of cancer, making them powerful early diagnostic markers. Lectin microarrays have become a powerful tool for both the study of glycosylation and the diagnosis of various diseases including cancer.Areas covered: This review will discuss the most useful features of lectin microarrays, such as their technological advances, their capability for parallel/high-throughput analysis for the important glycopatterns of glycoprotein, and an overview of their use for glycosylation analysis of various complex protein samples, as well as their diagnostic potential in various diseases.Expert opinion: Lectin microarrays have proved to be useful in studying multiple lectin-glycan interactions in a single experiment and, with the advances made in the field, hold a promise of enabling glycopatterns of diseases in a fast and efficient manner. Lectin microarrays will become increasingly powerful early diagnostic tool for a variety of conditions.

中文翻译：

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用于糖蛋白质组学的凝集素微阵列：其用途和潜力的概述。

简介：糖蛋白质组学是蛋白质组学的一个重要分支学科，重点研究蛋白质糖基化在各种生物过程中的作用。蛋白质糖基化是将糖或寡糖酶促添加到蛋白质上。改变的糖基化通常发生在疾病发展的早期阶段，例如，某些肿瘤相关聚糖已被证明在不同类型癌症的前体病变中表达，使它们成为强大的早期诊断标志物。凝集素微阵列已成为糖基化研究和包括癌症在内的各种疾病诊断的有力工具。涵盖的领域：本综述将讨论凝集素微阵列最有用的特征，例如它们的技术进步、它们对糖蛋白的重要糖型进行平行/高通量分析的能力，以及它们在各种复杂蛋白质样品的糖基化分析中的用途的概述，以及它们在各种疾病中的诊断潜力。 专家意见：凝集素微阵列已被证明是可用于在单个实验中研究多种凝集素 - 聚糖相互作用，并且随着该领域取得的进展，有望以快速有效的方式实现疾病的糖模式。凝集素微阵列将成为各种条件下越来越强大的早期诊断工具。凝集素微阵列已被证明可用于在单个实验中研究多种凝集素-聚糖相互作用，并且随着该领域取得的进展，有望以快速有效的方式实现疾病的糖模式。凝集素微阵列将成为各种条件下越来越强大的早期诊断工具。凝集素微阵列已被证明可用于在单个实验中研究多种凝集素-聚糖相互作用，并且随着该领域取得的进展，有望以快速有效的方式实现疾病的糖模式。凝集素微阵列将成为各种条件下越来越强大的早期诊断工具。