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Longitudinal study of rotavirus C VP6 genotype I6 in diarrheic piglets up to 1 week old
Brazilian Journal of Microbiology ( IF 2.1 ) Pub Date : 2020-01-30 , DOI: 10.1007/s42770-020-00234-z
Joice Elaine Teixeira Campanha 1 , Flávia Possatti 1 , Elis Lorenzetti 1, 2, 3 , Daniel de Almeida Moraes 1 , Alice Fernandes Alfieri 1, 2 , Amauri Alcindo Alfieri 1, 2
Affiliation  

The reports of rotavirus C (RVC) involvement in diarrhea outbreaks in newborn piglets have been increasing in recent years. This longitudinal study, conducted over a 37-day period, aimed to evaluate the frequency of RVC infection in piglets aged up to 7 days obtained from a pig herd with a previous diagnosis of RVC infection in this age group. Piglets from 50 different litters were monitored daily for the occurrence of diarrhea, and all litters were classified into the following categories: sow parity order (PO) 1 to 5; litter size (LS) ≤ 10 piglets and > 10 piglets; and piglet birth weight (BW) 1.2 to 1.3 kg and > 1.3 to 1.4 kg. Two hundred six diarrheic fecal samples were collected and classified according to the fecal consistency score (pasty, semiliquid, liquid). Ten fecal samples were collected from asymptomatic piglets (control group). Fecal samples were screened for rotavirus (RV) by silver stained-polyacrylamide gel electrophoresis (ss-PAGE), and samples with inconclusive and negative-ss-PAGE results were submitted to RVC VP6 gene amplification by RT-PCR. RVC was identified in 71 (34.5%) samples, in 1 (10%) sample of the control group, and in piglets from 33 (66%) litters. The electrophoretic profile of RV species A was identified in only two samples. Of the 72 RVC-positive samples, 51 (70.8%) presented semiliquid or liquid consistency. There was no significant difference in either group regarding the production parameters (PO, LS, BW) evaluated. An analysis of the whole VP6 gene of three RVC field strains collected on the first, fifteenth, and last day of the experiment enabled us to identify genotype I6. This report describes the first longitudinal study examining epidemiological aspects of RVC infection in newborn piglets.

中文翻译:

1周龄以下腹泻仔猪轮状病毒C VP6基因型I6的纵向研究

近年来,关于轮状病毒 C (RVC) 参与新生仔猪腹泻暴发的报道不断增加。这项纵向研究进行了 37 天,旨在评估从先前诊断为该年龄组 RVC 感染的猪群中获得的最大 7 天龄仔猪中 RVC 感染的频率。每天监测 50 窝不同窝仔猪的腹泻发生情况,所有窝均分为以下几类:母猪胎次 (PO) 1 至 5;窝产仔数 (LS) ≤ 10 头仔猪和 > 10 头仔猪;和仔猪出生体重 (BW) 1.2 至 1.3 公斤和 > 1.3 至 1.4 公斤。收集了 206 份腹泻性粪便样本,并根据粪便稠度评分(糊状、半液体、液体)进行分类。从无症状仔猪(对照组)收集十份粪便样本。通过银染聚丙烯酰胺凝胶电泳 (ss-PAGE) 筛选粪便样本中的轮状病毒 (RV),并将具有不确定和阴性 ss-PAGE 结果的样本通过 RT-PCR 进行 RVC VP6 基因扩增。在 71 个 (34.5%) 样本、1 个 (10%) 对照组样本和 33 (66%) 窝仔猪中发现了 RVC。仅在两个样品中鉴定了 RV 种类 A 的电泳图谱。在 72 个 RVC 阳性样品中,51 个 (70.8%) 呈现半液体或液体稠度。在评估的生产参数(PO、LS、BW)方面,两组没有显着差异。在实验的第一天、第十五天和最后一天收集的三个 RVC 田间菌株的整个 VP6 基因的分析使我们能够识别基因型 I6。
更新日期:2020-01-30
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