Introduction

Cell sheets consisting of adipose-derived stem cells (ADSCs) have been reported to be effective for wound healing. We conducted this study to clarify the efficacy of ADSC sheets in wound healing at the duct-to-duct biliary anastomotic site in pigs.

Methods

Eleven female pigs (20–25 kg) were divided into two groups: biliary anastomosis with an ADSC sheet (n = 6) or without an ADSC sheet (n = 5). To follow the transplanted ADSCs, PKH26GL-labeled sheets were used in one of the ADSC pigs. Two weeks prior to laparotomy, ADSCs were isolated from the lower abdominal subcutaneous adipose tissue. After three passages, ADSCs were seeded on temperature-responsive culture dishes and collected as cell sheets. ADSC sheets were gently transplanted on the anastomotic site. We evaluated specimens by PKH26GL labeling, macroscopic changes, infiltration of inflammatory cells, and collagen content.

Results

Labeled ADSCs remained around the bile duct wall. In the no-ADSC group, more adhesion developed at the hepatic hilum as observed during relaparotomy. Histopathological examination showed that the diameter and cross-sectional area of the bile duct wall were decreased in the ADSC group. In the no-ADSC group, a large number of inflammatory cells and more collagen fibers were identified in the bile duct wall.

Conclusions

The present study demonstrated that autologous ADSC sheet transplantation reduced hypertrophic changes in the bile duct wall at the anastomotic site. A long-term follow-up is required to evaluate the efficacy of this mechanism in prevention of biliary anastomotic strictures.

中文翻译：

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通过自体脂肪干细胞片改善胆道吻合的愈合。

介绍

据报道，由脂肪干细胞 (ADSC) 组成的细胞片对伤口愈合有效。我们进行了这项研究，以阐明 ADSC 片在猪的管对管胆道吻合部位伤口愈合中的功效。

方法

11 头雌性猪 (20-25 kg) 被分为两组：有 ADSC 表的胆道吻合 (n = 6) 或没有 ADSC 表 (n = 5)。为了跟踪移植的 ADSC，在其中一只 ADSC 猪中使用了 PKH26GL 标记的薄片。在剖腹手术前两周，从下腹部皮下脂肪组织中分离出 ADSC。三代后，将 ADSC 接种在温度响应培养皿上并收集为细胞片。将 ADSC 片材轻轻移植到吻合部位。我们通过 PKH26GL 标记、宏观变化、炎症细胞浸润和胶原蛋白含量来评估标本。

结果

标记的 ADSC 保留在胆管壁周围。在无 ADSC 组中，如再剖腹术中观察到的，在肝门处出现了更多的粘连。组织病理学检查显示ADSC组胆管壁直径和横截面积减小。在非 ADSC 组中，胆管壁中发现了大量的炎症细胞和更多的胶原纤维。

结论

本研究表明，自体 ADSC 片移植减少了吻合部位胆管壁的肥大变化。需要长期随访来评估这种机制在预防胆道吻合口狭窄方面的功效。