The cytoskeleton of eukaryotic cells contains networks of actin filaments and microtubules (MTs) that are jointly implicated in various cell functions, including cell division, morphogenesis, and migration. In neurons, this synergistic activity drives both the formation of axons during development and synaptic activity in mature neurons. Both actin filaments and MTs also are highly charged polyelectrolytes that generate and conduct electrical signals. However, no information is presently available on a potential electrical crosstalk between these two cytoskeletal networks. Herein we tested the effect of actin polymerization on the electrical oscillations generated by two‐dimensional sheets of bovine brain microtubule protein (2D‐MT). The voltage‐clamped 2D‐MT sheets displayed spontaneous electrical oscillations representing a synchronous 224% change in conductance, and a fundamental frequency of 38 Hz. At 60 mV, a 4.15 nC of integrated charge transferred per second increased by 72.3% (7.15 nC) after addition of monomeric (G)‐actin. This phenomenon had a 2‐min lag time, and was prevented by the presence of the G‐actin‐binding protein DNAse I. Addition of prepolymerized F‐actin, however, had a rapid onset (<10 s) and a higher effect on the tubulin sheets (~100% increase, 8.25 nC). The data are consistent with an interaction between the actin cytoskeleton and tubulin structures, in what seems to be an electrostatic effect. Because actin filaments and MTs interact with each other in neurons, it is possible for this phenomenon to be present, and of relevance in the processing of intracellular signaling, including the gating and activation of actin cytoskeleton‐regulated excitable ion channels in neurons.

中文翻译：

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肌动蛋白丝调节大脑微管蛋白二维片的电活动。

真核细胞的细胞骨架包含肌动蛋白丝和微管（MTs）网络，这些网络共同参与各种细胞功能，包括细胞分裂，形态发生和迁移。在神经元中，这种协同活性既可驱动发育过程中轴突的形成，又可驱动成熟神经元中的突触活性。肌动蛋白丝和MT都是带高电荷的聚电解质，可产生并传导电信号。但是，目前尚无有关这两个细胞骨架网络之间潜在电串扰的信息。在这里，我们测试了肌动蛋白聚合对二维二维牛脑微管蛋白（2D-MT）产生的电振荡的影响。电压钳制的2D-MT工作表显示出自发的电振荡，表示电导同步变化224％，基频为38 Hz。添加单体（G）-肌动蛋白后，在60 mV时，每秒传输的4.15 nC积分电荷增加了72.3％（7.15 nC）。这种现象有2分钟的滞后时间，并且可以通过G-肌动蛋白结合蛋白DNAse I的存在来防止。但是，预聚合的F-肌动蛋白的添加起效快（<10 s），并且对G的作用更大。微管蛋白片（增加约100％，8.25 nC）。数据与肌动蛋白细胞骨架和微管蛋白结构之间的相互作用一致，这似乎是静电作用。由于肌动蛋白丝和MT在神经元中相互作用，因此可能会出现这种现象，