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Higher levels of TNF and IL-4 cytokines and low miR-182 expression in visceral leishmaniasis-HIV co-infected patients.
Parasite Immunology ( IF 1.4 ) Pub Date : 2020-03-17 , DOI: 10.1111/pim.12701
Walter Lins Barbosa Júnior 1 , Alda Maria Justo 2 , Ana Maria Aguiar Dos Santos 1 , Virginia Maria Barros de Lorena 3 , Rodrigo Feliciano do Carmo 4 , Fábio Lopes de Melo 1 , Zulma Maria de Medeiros 1, 2 , Luydson Richardson Silva Vasconcelos 1, 2
Affiliation  

AIMS The aim of the present study was to assess serum cytokine and miRNA expression in visceral leishmaniasis-HIV (VL-HIV) co-infection and HIV mono-infection. METHODS AND RESULTS We analyzed 113 serum samples from HIV patients in areas endemic for leishmaniasis. The diagnosis of VL was confirmed in 65 of these 113 samples. The VL-HIV and HIV groups presented significant differences regarding hemoglobin level (p < 0.0001), lymphocyte count (p = 0.0444), white blood cell count (p = 0.0108), weight loss (p = 0.0310), HIV load (p < 0.0001) and CD4+ T-lymphocytes count (p = 0.0003). Levels of IL-6 and IL-10, IFN-γ and IL-6, IFN-γ and IL-10, TNF and IL-2 were positively correlated in VL-HIV co-infection, indicating higher serum levels of TNF and IL-4 (p < 0.0001). In addition, miR-182 expression was found to be significantly higher in HIV (p = 0.009), miR-210 exhibited no statistically significant difference between groups, and non-expression of miR-122 was found in both groups. CONCLUSION Together, TNF, IL-4 and miR-182 may represent circulatory biomarkers of VL-HIV co-infection.

中文翻译:

内脏利什曼病-HIV合并感染患者的TNF和IL-4细胞因子水平较高,miR-182表达水平较低。

目的本研究的目的是评估内脏利什曼病-HIV(VL-HIV)合并感染和HIV单一感染中的血清细胞因子和miRNA表达。方法和结果我们分析了利什曼病流行地区的113例HIV患者的血清样本。在这113个样本中的65个样本中证实了VL的诊断。VL-HIV和HIV组在血红蛋白水平(p <0.0001),淋巴细胞计数(p = 0.0444),白细胞计数(p = 0.0108),体重减轻(p = 0.0310),HIV负荷(p < 0.0001)和CD4 + T淋巴细胞计数(p = 0.0003)。VL-HIV合并感染中IL-6和IL-10,IFN-γ和IL-6,IFN-γ和IL-10,TNF和IL-2的水平呈正相关,表明血清TNF和IL的水平较高-4(p <0.0001)。此外,发现miR-182表达在HIV中显着更高(p = 0.009),miR-210在各组之间没有统计学上的显着差异,并且在两组中均未发现miR-122的表达。结论TNF,IL-4和miR-182共同代表了VL-HIV合并感染的循环生物标志物。
更新日期:2020-03-27
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