Thyroid cancer (TC) has become one of most common endocrine malignancies in recent decades. Due to gene background polymorphism, it's outcome goes quite differently in each patient. For exploring the mechanism, we performed whole transcriptome sequencing of paired papillary thyroid carcinoma (PTC) and adjacent thyroid tissues. As a result, scavenger receptor class A member 5 (SCARA5) might be a crucial anti-oncogene associated with PTC. By RT-qPCR, we first detected the expression of SCARA5 in PTC tissue and three type of TC cell lines. Besides, The Cancer Genome Atlas (TCGA) data were gathered to analysis the relationship between SCARA5 and clinical feature. A series of loss-function experiments in TC cell lines (KTC-1 and BCPAP) to investigate the function of SCARA5 in PTC. The results showed that SCARA5 expression in PTC was lower than adjacent normal tissue. And, it's consistent with the TCGA database. After analyse the correlation between SCARA5 expression and clinicopathological features in TCGA database, we discovered that downregulated SCARA5 is significantly connected age (P = .04) and tumour size (P = .032). Knockdown of SCARA5 in TC cell line could significantly increase the function of cells proliferation, colony formation, migration, and invasion. Furthermore, we also proved that SCARA5 could modulate the expression of epithelial-mesenchymal transition-related proteins, which influence invasion and migration. To best of our knowledge, SCARA5 is a suppressor gene which was associated with PTC and might be a potential therapeutic target in the future. SIGNIFICANCE OF THE STUDY: Thyroid cancer (TC) has become one of most common endocrine malignancies in recent decades. By whole transcriptome sequencing of paired papillary thyroid carcinoma (PTC) and adjacent thyroid tissues, author discovered that scavenger receptor class A member 5 (SCARA5) might be crucial anti-oncogene associated with PTC. Furthermore, knocking-down of SCARA5 in TC cell line can increase the function of cells proliferation, colony formation, migration, and invasion. Author also proved that SCARA5 could modulate the expression of epithelial-mesenchymal transition-related proteins.

中文翻译：

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清道夫受体A类，成员5与甲状腺癌细胞系通过上皮-间质转化相关。

甲状腺癌（TC）已成为近几十年来最常见的内分泌恶性肿瘤之一。由于基因背景多态性，每个患者的结局都大不相同。为了探索该机制，我们对配对的乳头状甲状腺癌（PTC）和邻近的甲状腺组织进行了全转录组测序。因此，清道夫受体A类5成员（SCARA5）可能是与PTC相关的关键抗癌基因。通过RT-qPCR，我们首先检测了SCARA5在PTC组织和三种类型的TC细胞系中的表达。此外，收集了癌症基因组图谱（TCGA）数据以分析SCARA5与临床特征之间的关系。在TC细胞系（KTC-1和BCPAP）中进行了一系列损失功能实验，以研究SCARA5在PTC中的功能。结果表明，PTC中的SCARA5表达低于邻近的正常组织。并且，它与TCGA数据库一致。在分析TCGA数据库中SCARA5表达与临床病理特征之间的相关性之后，我们发现下调的SCARA5与年龄（P = .04）和肿瘤大小（P = .032）有显着联系。敲低TC细胞系中SCARA5可以显着增加细胞增殖，集落形成，迁移和侵袭的功能。此外，我们还证明了SCARA5可以调节上皮-间质转化相关蛋白的表达，从而影响侵袭和迁移。据我们所知，SCARA5是与PTC相关的抑制基因，将来可能成为潜在的治疗靶标。学习的重点：甲状腺癌（TC）已成为近几十年来最常见的内分泌恶性肿瘤之一。通过对成对的乳头状甲状腺癌（PTC）和邻近的甲状腺组织进行全转录组测序，作者发现清道夫受体A类5成员（SCARA5）可能是与PTC相关的关键抗癌基因。此外，在TC细胞系中敲除SCARA5可以增加细胞增殖，集落形成，迁移和侵袭的功能。作者还证明了SCARA5可以调节上皮-间质转化相关蛋白的表达。作者发现清道夫受体A类5成员（SCARA5）可能是与PTC相关的关键抗癌基因。此外，在TC细胞系中敲除SCARA5可以增加细胞增殖，集落形成，迁移和侵袭的功能。作者还证明了SCARA5可以调节上皮-间质转化相关蛋白的表达。作者发现清道夫受体A类5成员（SCARA5）可能是与PTC相关的关键抗癌基因。此外，在TC细胞系中敲除SCARA5可以增加细胞增殖，集落形成，迁移和侵袭的功能。作者还证明了SCARA5可以调节上皮-间质转化相关蛋白的表达。