In recent years, emerging evidence has illustrated the indispensable role of sympathetic neurotransmitters and their receptors in cartilage mediation. The presence of neuropeptide Y (NPY)-positive sympathetic nerve fibres in cartilage and NPY-secretion function in chondrocytes raises the possibility of NPY directly regulating the function of chondrocytes. Therefore, this study intended to evaluate the effect of NPY and its receptors on the proliferation and chondrogenic differentiation of ATDC5 cells. Results showed NPY, especially at a concentration of 10-10 M, to significantly enhance proliferation of ATDC5 cells. Moreover, NPY effectively facilitated early chondrogenesis and late hypertrophy/mineralisation of ATDC5 cells via Y1 receptor signalling, rather than via Y2 receptor signalling. Taken together, the results help us to understand how NPY and its receptors affect the function of chondrocytes.

中文翻译：

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神经肽Y增强ATDC5细胞的增殖和软骨分化

近年来，新出现的证据说明了交感神经递质及其受体在软骨介导中不可或缺的作用。软骨中神经肽 Y (NPY) 阳性交感神经纤维和软骨细胞中 NPY 分泌功能的存在提高了 NPY 直接调节软骨细胞功能的可能性。因此，本研究拟评估NPY及其受体对ATDC5细胞增殖和软骨分化的影响。结果显示 NPY，尤其是在 10-10 M 的浓度下，可显着增强 ATDC5 细胞的增殖。此外，NPY 通过 Y1 受体信号传导而不是通过 Y2 受体信号传导有效促进 ATDC5 细胞的早期软骨形成和晚期肥大/矿化。综合起来，