Studies on Sisorinae systematics have been largely restricted to morphological data with few studies on examination of phylogenetic relations. However, no study has been done to evaluate genetic distance of the genera under Sisorinae sub-family and detailed phylogenetic relations within it. We used nuclear recombination activating 2 (rag2) gene and mitochondrial cytochrome c oxidase I (COI) gene from 64 species to examine genetic differentiation and phylogenetic relationships within 11 Asian Sisorinae genera. The range of interspecies K2P distance for rag2 was 0-0.061 and COI was 0-0.204. Phylogenetic analysis based on maximum likelihood (ML) and Bayesian (BI) approaches for each locus individually and for the concatenated rag2 and COI sequences revealed three major subclades viz. Bagariini, Sisorini and Erethistini under subfamily Sisorinae. The analysis based on COI gene showed ((Sisorini, Bagariini), Erethistini) relationship. Rag2 and combined rag2 and COI showed ((Sisorini, Erethistini), Bagariini) relationship. Combined rag2 and COI analyses resulted into better resolved trees with a good bootstrap support. In this study, new record of Pseudolaguvia foveolata (Erethistini) has been documented based on 13 specimens collected from Torsa River, Jaldapara, Alipurduar district, West Bengal, India (26°43'44.66″ N and 89°19'32.34″ E), extending its distribution range in Brahmaputra drainage, India. The genetic distance between the P. foveolata new record and the reported P. foveolata (holotype: UMMZ 244867) was 0.00 at both rag2 and COI locus and it was further grouped with P. foveolata Type specimen (holotype: UMMZ 244867) with 100% bootstrap support. This report gives additional information on occurrence of the species P. foveolata, along with discussion on morphometric, meristic and molecular (COI and rag2 gene) data.

中文翻译：

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11个亚洲Sisorinae属（Siluriformes：Sisoridae）的遗传分化和系统发育关系与新的假单胞菌（Pseudolaguvia foveolata）记录有关。

对Sisorinae系统学的研究在很大程度上局限于形态学数据，很少有关于系统发育关系检查的研究。但是，尚未进行评估Sisorinae亚科下属的遗传距离及其内部详细系统发育关系的研究。我们使用核重组激活2（rag2）基因和线粒体细胞色素C氧化酶I（COI）基因来自64个物种，以检查11个亚洲Sisorinae属中的遗传分化和系统发育关系。rag2的种间K2P距离范围为0-0.061，COI为0-0.204。对每个基因座以及串联的rag2和COI序列分别基于最大似然（ML）和贝叶斯（BI）方法进行的系统发育分析揭示了三个主要子分支。巴索里尼，西索里尼和埃雷斯蒂斯尼隶属于西索里娜亚家族。基于COI基因的分析显示了（（Sisorini，Bagariini），Erethistini）的关系。Rag2以及rag2和COI的组合显示（（Sisorini，Erethistini），Bagariini）关系。结合了rag2和COI分析，可以得到具有良好引导程序支持的更好解析的树。在这项研究中，根据从印度西孟加拉邦阿里普杜尔区贾尔达帕拉的托尔萨河采集的13个标本（26°43'44.66“ N和89°19'32.34” E）记录了新的假单胞菌（Esehistini）记录。 ，扩展了其在印度Brahmaputra排水系统中的分销范围。在rag2和COI位点上，新发现的小叶黄杨与记录的小叶黄杨（遗传型：UMMZ 244867）之间的遗传距离均为0.00，并且进一步与100％的小叶黄萎病类型标本（分型：UMMZ 244867）分组引导程序支持。