Bovine serum albumin (BSA)-coated haemoglobin (Hb)-microcapsules prepared by co-precipitation of Hb and MnCO3 may present an alternative type of artificial blood substitute. Prepared microcapsules were analysed by Scanning electron microscopy (SEM) and Respirometry, cytotoxicity was evaluated by addition of microcapsules to murine fibroblast-derived cell line L929 (American Type Culture Collection, NCTC clone 929 of strain L). The capsules come along with a mean diameter of approximately 0.6 μm and a mean volume of 1.13 × 10-19 L, thus an average human red blood cell with a volume of 9 × 10-14 L is about 800,000 times bigger. Hb-microcapsules are fully regenerable by ascorbic acid and maintain oxygen affinity because oxygen is able to pass the BSA wall of the capsules and thereby binding to the ferrous iron of the haemoglobin entity. Therefore, these microcapsules present a suitable type of potential artificial haemoglobin-based oxygen carrier (HbOC).

中文翻译：

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Genipin交联的白蛋白包被的基于血红蛋白的水性微胶囊的合成与表征。

通过共沉淀Hb和MnCO3制备的牛血清白蛋白（BSA）包被的血红蛋白（Hb）-微胶囊可能是人工血液替代品的另一种类型。通过扫描电子显微镜（SEM）和呼吸测定法分析制备的微胶囊，通过向鼠成纤维细胞衍生的细胞系L929（美国典型培养物保藏中心，菌株L的NCTC克隆929）中添加微胶囊来评价细胞毒性。胶囊的平均直径约为0.6μm，平均体积为1.13×10-19 L，因此，平均人类红细胞的体积为9×10-14 L约大80万倍。Hb微胶囊可通过抗坏血酸完全再生，并保持氧气亲和力，因为氧气能够通过胶囊的BSA壁，从而与血红蛋白实体的亚铁结合。因此，