Previous RNA-Seq analyses revealed that NAD(P)H steroid dehydrogenase-like (NSDHL) has a different expression during 3T3-L1 differentiation; however, its roles in adipogenesis are unknown. In the present study, using quantitative real-time PCR, we confirmed that NSDHL knockdown increased the proliferation of 3T3-L1 preadipocytes, but attenuated the differentiation of 3T3-L1 preadipocytes, as evidenced by reduced lipid accumulation and down-regulation of PPARγ gene expression. Further analyses showed that the expression peak of NSDHL was at the early stage of 3T3-L1 preadipocytes differentiation and LXR-SREBP1 signaling pathway was downregulated in NSDHL-knockdown 3T3-L1 cells. Collectively, our findings indicate that NSDHL is a novel modulator of adipogenesis. Moreover, our data provide insight into the complex relationships between sterol sensing, LXR-SREBP1 signaling pathway, and PPARγ in 3T3-L1 cells.

中文翻译：

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NSDHL的抑制通过3T3-L1细胞中LXR-SREBP1途径的下调来减弱脂肪形成。

先前的RNA-Seq分析显示，NAD（P）H类固醇脱氢酶样（NSDHL）在3T3-L1分化过程中具有不同的表达；然而，其在脂肪形成中的作用尚不清楚。在本研究中，我们使用定量实时PCR证实了NSDHL抑制可增加3T3-L1前脂肪细胞的增殖，但减弱了3T3-L1前脂肪细胞的分化，这通过减少脂质积累和下调PPARγ基因表达来证明。 。进一步的分析表明，NSDHL的表达高峰是在3T3-L1前脂肪细胞分化的早期，并且在NSDHL-knockdown 3T3-L1细胞中LXR-SREBP1信号通路被下调。总的来说，我们的发现表明NSDHL是一种新型的脂肪形成调节剂。此外，我们的数据可洞悉固醇感测之间的复杂关系，