The aim of this study was to establish a model to induce cystic ovarian follicles (COFs) in cattle using the cyclooxygenase inhibitor, indomethacin. Eighteen Holstein-Frisian cattle were synchronized with prostaglandin F2alpha (PGF2α) and gonadotropin-releasing hormone (GnRH). Ultrasound-guided transvaginal intrafollicular injections were performed in 23 preovulatory follicles with different concentrations of indomethacin 16 h after GnRH administration. An injection of 0.2 ml 35 µM indomethacin solution (resulting in a final concentration of 8 µg/ml in the follicular fluid) was the minimal dosage leading to COF formation. The induced COFs reached a maximum mean diameter of 36.9 ± 4.5 mm eleven days after injection. The estrous cycle was extended to 25–39 days. Luteinization was first observed 4 days after injection, accompanied by a slight increase in plasma progesterone concentration. The bioactivity of indomethacin was demonstrated by the decrease of prostaglandin E2 in the follicular fluid of three animals. The method presented here is minimally invasive and allows for the generation of defined COFs for further investigations.

中文翻译：

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通过卵泡内注射吲哚美辛诱导牛囊性卵巢卵泡（COF）


本研究的目的是建立一个使用环氧合酶抑制剂吲哚美辛诱导牛囊性卵巢卵泡 (COF) 的模型。十八头荷斯坦-弗里斯兰牛与前列腺素 F2α (PGF2α) 和促性腺激素释放激素 (GnRH) 同步。 GnRH给药16 h后，在超声引导下经阴道卵泡内注射不同浓度的吲哚美辛，对23个排卵前卵泡进行。注射 0.2 ml 35 µM 吲哚美辛溶液（卵泡液中最终浓度为 8 µg/ml）是导致 COF 形成的最小剂量。注射后 11 天，诱导的 COF 达到最大平均直径 36.9 ± 4.5 mm。发情周期延长至25-39天。注射后 4 天首次观察到黄体化，同时血浆孕酮浓度略有增加。通过三只动物卵泡液中前列腺素 E2 的减少证明了吲哚美辛的生物活性。这里介绍的方法是微创的，可以生成定义的 COF 以供进一步研究。