Approaches based on association studies have proven useful in identifying genetic predictors for many diseases, including susceptibility to chronic hepatitis B. In this study we were interested by the IL‐1B genetic variants that have been involved in the immune response and we analyzed their role in the susceptibility to develop chronic hepatitis B in the Tunisian population. IL‐ 1B is a potent proinflammatory cytokine that plays an important role in inflammation of the liver. Polymorphic gene IL‐1 (− 511, +3954) was analyzed in a total of 476 individuals: 236 patients with chronic hepatitis B from different cities of Tunisia recruited in Pasteur Institute between January 2017 and December 2018 and 240 controls. Genomic DNA was obtained using the standard salting‐out method and genotyping was performed by polymerase chain reaction (PCR)‐restriction fragment length polymorphism. For − 511C>T polymorphism a significant association was found between patients and controls when comparing the genotypic (P  = 0.007; χ² = 9.74 and odds ratio [OR] = 0.60; confidence interval [CI] = 0.41–0.89) and allelic (P  = 0.001; χ² = 10.60) frequencies. When the viral load was taken into account a highly significant difference was found (P  = 9 × 10⁻⁴; χ² = 10.89). For +3954C>T polymorphism a significant association was found between patients and controls when comparing genotypic (P  = 0.0058; χ² = 7.60 and OR = 1.67; CI = 1.14–2.46) and allelic (P  = 0.0029; χ² = 8.81) frequencies. T allele can be used as a strong marker for hepatitis B virus disease for both polymorphisms.

中文翻译：

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突尼斯慢性乙型肝炎患者白细胞介素IL-1B基因多态性：与复制水平的关联。

已证明基于关联研究的方法可用于确定许多疾病的遗传预测因子，包括对慢性乙型肝炎的易感性。在这项研究中，我们对参与免疫应答的IL-1B基因变异感兴趣，我们分析了它们在免疫反应中的作用。突尼斯人口中慢性乙型肝炎的易感性。IL - 1B是有效的促炎细胞因子，在肝脏炎症中起重要作用。多态性基因IL-1（-511，+3954）在总共476人中进行了分析：2017年1月至2018年12月在巴斯德研究所招募的来自突尼斯不同城市的236例慢性乙型肝炎患者和240名对照。使用标准的盐析方法获得基因组DNA，并通过聚合酶链反应（PCR）-限制性片段长度多态性进行基因分型。对于- 511C> T多态性一个显著关联发现患者和比较的基因型时对照之间（P  = 0.007;χ ²  = 9.74和比值比[OR] = 0.60;置信区间[CI] = 0.41-0.89）和等位基因（P  = 0.001;χ ² = 10.60）的频率。当病毒载量考虑到一个高度显著差异（P  = 9×10 ^-4 ;χ ²  = 10.89）。对于+ 3954C> T多态性的基因型进行比较，当显著相关性患者和对照之间实测值（P  = 0.0058;χ ²  = 7.60和OR = 1.67; CI = 1.14-2.46）和等位基因（P  = 0.0029;χ ²  = 8.81）频率。T等位基因可以用作两种多态性的乙型肝炎病毒疾病的强标志物。