BACKGROUND Surface nanostructures in titanium (Ti) oral implants are critical for rapid osseointegration. OBJECTIVE The purpose of this study was to evaluate the growth of osteoblast-like (Saos-2) and epithelial-like (Ca9-22) cells on nanopatterned Ti films. METHODS Ti films with 500 nm grooves and pillars were fabricated by nanoimprinting, and seeded with Saos-2 and Ca9-22 cells. Cell viability and morphology were assessed by cell proliferation assay and scanning electron microscopy, respectively. RESULTS As assessed after 1 hour, proliferation of Saos-2 cells was most robust on grooved films than on pillared and smooth films, in this order. These cells approximately doubled on grooved and pillared substrates in 24 hours and after 5 days, but not on smooth surfaces. In contrast, Ca9-22 cells favored smooth surfaces, followed by grooved and pillared films. Indeed, cells sparsely adhered to pillared films over 5 days of incubation (p < 0.05). CONCLUSIONS The data show that Saos-2 and Ca9-22 cells respond differently to different nanostructures, and highlight the potential use of nanopatterns to promote bone regeneration or to prevent epithelial downgrowth at the implant-bone interface.

中文翻译：

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开槽和带柱子的钛表面上Saos-2和Ca9-22细胞的增殖。

背景技术钛（Ti）口腔植入物中的表面纳米结构对于快速骨整合至关重要。目的本研究的目的是评估纳米图案Ti膜上成骨样（Saos-2）和上皮样（Ca9-22）细胞的生长。方法通过纳米压印法制备具有500nm沟槽和柱状的Ti膜，并在其上植入Saos-2和Ca9-22细胞。通过细胞增殖测定法和扫描电子显微镜分别评价细胞活力和形态。结果1小时后评估，开槽膜上的Saos-2细胞的增殖比起柱状和光滑膜上的增殖顺序最强。这些电池在24小时内和5天后在开槽和带柱状的基材上大约增加了一倍，但在光滑的表面上却没有。相比之下，Ca9-22细胞更喜欢光滑的表面，其次是开槽和带柱子的薄膜。确实，在孵育5天后，细胞稀疏地粘附在有柱膜上（p <0.05）。结论数据显示Saos-2和Ca9-22细胞对不同的纳米结构有不同的反应，并强调了使用纳米模式促进骨再生或防止植入物-骨界面上皮下生长的潜在用途。