External fixators of serious fractures could be an attractive substrate on which microorganisms can accumulate. Therefore, this study aimed to develop a suitable method for enabling the simulation of a real situation when osteosynthetic fixation material is open for the potential threat of bacterial attack. Agar-based media represented human tissue, and the metallic pin characterized the screw in the fixation. Various types of agar, supplements, and contamination strategy by Staphylococcus aureus were tested. The influence of the initial bacterial concentration was also examined. Surfaces were observed by scanning electron microscopy (SEM), and all results were compared. Brain Heart Infusion Agar with the Egg Yolk Tellurite Emulsion was established in a transparent test tube as a suitable system for enabling the good interpretability of bacterial contamination in the pin’s surroundings. Pin contamination has been found to be an appropriate approach for testing microbial growth, rather than agar surface contamination, which distorted obtained results. A lower initial colony forming units (CFU) provided better clarity of the test. SEM observation of the pin surface was comparable with the visual evaluations in the test tubes. Results were assembled for positive and negative control samples as well. Screening method for the most common bacteria S. aureus has been standardized and developed. This experimental setup could also be a useful tool for surface modification with antibacterial properties testing.

严重骨折的外固定器可能是吸引微生物的基质。因此，本研究旨在开发一种合适的方法，以在骨合成固定材料对细菌攻击的潜在威胁开放时能够模拟真实情况。琼脂基培养基代表了人体组织，而金属针则是固定螺钉的特征。金黄色葡萄球菌的各种琼脂，补充剂和污染策略经过测试。还检查了初始细菌浓度的影响。通过扫描电子显微镜（SEM）观察表面，并比较所有结果。在透明试管中建立了带有蛋黄碲酸盐乳剂的脑心浸液琼脂，作为合适的系统，可以很好地解释图钉周围环境中的细菌污染。已经发现，针污染是测试微生物生长的合适方法，而不是琼脂表面污染，这会使获得的结果失真。较低的初始菌落形成单位（CFU）提供了更好的测试清晰度。针表面的SEM观察结果与试管中的视觉评估结果相当。收集阳性和阴性对照样品的结果。金黄色葡萄球菌已经标准化和开发。该实验设置也可能是通过抗菌性能测试进行表面改性的有用工具。