Purpose

Sarcocystis spp. are protozoan parasites of livestock which also infect birds, lower vertebrates and mammals, including man. Wild and domestic ruminants such as red deer, roe deer, fallow deer, cattle, sheep and goats may act as intermediate hosts for many Sarcocystis species, some of which are significant pathogens causing sarcocystosis in livestock and humans. The purpose of the present study was to determine the prevalence of Sarcocystis species in fallow deer farmed in an open pasture system.

Methods

Samples of heart and oesophagus tissue taken from five fallow deer were examined by light microscope for the presence of sarcocysts. Genomic DNA was extracted from individual sarcocysts. ssu rRNA was successfully amplified using their DNA as templates.

Results

Analysis of the ssu rRNA identified the presence of two S. morae sarcocysts in the heart tissue; similarly, S. gracilis sarcocysts were identified in the heart and oesophagus, and Sarcocystis sp. most closely related to S. linearis and S. taeniata were detected in oseophagus.

Conclusions

These findings confirm the presence of Sarcocystis spp. in farmed fallow deer in Poland; however, more molecular studies are needed.

中文翻译：

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基于ssu rRNA基因在开放式牧场系统中养殖的小鹿（Dama dama）组织的囊肿的分子鉴定。

目的

肉囊藻属 是牲畜的原生动物寄生虫，也感染鸟类，低等脊椎动物和哺乳动物，包括人。野生和家养反刍动物，如马鹿，ro，小鹿，牛，绵羊和山羊，可能是许多石藻种类的中间宿主，其中一些是引起牲畜和人类结石病的重要病原体。本研究的目的是确定在开放式牧场系统中养殖的小鹿中石藻种类的流行程度。

方法

通过光学显微镜检查了从五只小鹿采集的心脏和食道组织样品中是否有石藻。从单个的卵囊提取基因组DNA。使用其DNA作为模板成功扩增了ssu rRNA。

结果

对ssu rRNA的分析确定了心脏组织中存在两个桑蚕丝囊藻卵囊。同样地，在心脏和食道中发现了S. gracilis肉囊肿，并且在肉囊中发现了Sarcocystis sp。最密切相关的S.线形和S. taeniata在oseophagus进行检测。

结论

这些发现证实了Sarcocystis spp的存在。在波兰养殖的小鹿中；但是，需要更多的分子研究。