Vitellogenin receptor (VgR) mediates the intake of vitellin via oocytes, thus exerting an important role in vitellogenesis. In this study, reverse transcription-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends techniques were adopted to clone the CiVgR gene, namely the VgR gene of Calliptamus italicus, i.e., Orthopteran. The full length of CiVgR was 5,589 bp, and the open reading frame was estimated to be 5,265 bp, which encoded 1,754 amino acids (aa). Sequence alignment analysis showed that CiVgR belonged to the superfamily of low-density lipoprotein receptor genes, which contained several conserved domains, including ligand-binding domains, epidermal growth factor precursor homology domains, transmembrane domains, and cytoplasmic domains. However, no O-linked sugar domain was identified. Phylogenetic analysis showed that CiVgR had the closest genetic relationship to Blattarias. RT-PCR showed that CiVgR was only specifically expressed in the ovarian tissue of females. quantitative real time polymerase chain reaction showed that the transcription of CiVgR already appeared in the fourth-instar nymph of C. italicus, which gradually increased after adult emergence, peaked at the previtellogenesis stage, and then started to decrease. The expression pattern of CiVgR was closely associated with vitellogenesis. The findings of this study further our understanding of the molecular mechanisms involved in the reproduction of C. italicus, and provide new ideas to control this insect.

中文翻译：

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意大利Call鱼（直翅目：in科）中卵黄蛋白原受体的分子特征和表达模式。

卵黄蛋白原受体（VgR）通过卵母细胞介导卵黄蛋白的摄取，因此在卵黄发生中起重要作用。在这项研究中，采用逆转录聚合酶链反应（RT-PCR）和cDNA末端快速扩增技术克隆了CiVgR基因，即意大利Calliptamus italicus的VgR基因，即直翅目。CiVgR的全长为5589 bp，开放阅读框估计为5265 bp，编码1,754个氨基酸（aa）。序列比对分析表明，CiVgR属于低密度脂蛋白受体基因的超家族，其包含几个保守的结构域，包括配体结合结构域，表皮生长因子前体同源结构域，跨膜结构域和细胞质结构域。然而，未鉴定出O-连接的糖结构域。系统发育分析表明，CiVgR与布拉氏菌的亲缘关系最近。RT-PCR显示CiVgR仅在女性的卵巢组织中特异性表达。定量实时聚合酶链反应显示，CiVgR的转录已经出现在意大利纤毛虫的四龄若虫中，其在成虫出现后逐渐增加，在前卵形成期达到顶峰，然后开始减少。CiVgR的表达模式与卵黄发生密切相关。这项研究的发现进一步加深了我们对意大利线虫繁殖的分子机制的理解，并为控制这种昆虫提供了新的思路。定量实时聚合酶链反应显示，CiVgR的转录已经出现在意大利纤毛虫的四龄若虫中，其在成虫出现后逐渐增加，在前卵形成期达到顶峰，然后开始减少。CiVgR的表达模式与卵黄发生密切相关。这项研究的发现进一步加深了我们对意大利线虫繁殖的分子机制的理解，并为控制这种昆虫提供了新的思路。定量实时聚合酶链反应显示，CiVgR的转录已经出现在意大利纤毛虫的四龄若虫中，其在成虫出现后逐渐增加，在前卵形成期达到顶峰，然后开始减少。CiVgR的表达模式与卵黄发生密切相关。这项研究的发现进一步加深了我们对意大利线虫繁殖的分子机制的理解，并为控制这种昆虫提供了新的思路。